DICP OpenIR
Subject Area物理化学
An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome
Bian, Yangyang1,2; Song, Chunxia1,2; Cheng, Kai1,2; Dong, Mingming1,2; Wang, Fangjun1; Huang, Junfeng1,2; Sun, Deguang3; Wang, Liming3; Ye, Mingliang1; Zou, Hanfa1; Ye ML(叶明亮); Zou HF(邹汉法)
KeywordHuman Liver Phosphoproteome Rp-rplc Glu-c Digestion Tripletof 5600 Ltq Orbitrap Velos Mass Spectrometer
Source PublicationJOURNAL OF PROTEOMICS
2014-01-16
ISSN1874-3919
DOI10.1016/j.jprot.2013.11.014
Volume96Pages:253-262
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1809
Contribution Rank待补充
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordION AFFINITY-CHROMATOGRAPHY ; PROTEIN IDENTIFICATION TECHNOLOGY ; PHOSPHORYLATED PEPTIDES ; MASS-SPECTROMETRY ; MULTIDIMENSIONAL SEPARATION ; ENRICHMENT ; PROTEOMICS ; PHOSPHOPEPTIDES ; STRATEGY ; NETWORKS
AbstractProtein phosphorylation is one of the most common post-translational modifications. It plays key roles in regulating diverse biological processes of liver tissues. To better understand the role of protein phosphorylation in liver functions, it is essential to perform in-depth phosphoproteome analysis of human liver. Here, an enzyme assisted reversed-phase-reversed-phase liquid chromatography (RP-RPLC) approach with both RPLC separations operated with optimized acidic mobile phase was developed. High orthogonal separation was achieved by trypsin digestion of the Glu-C generated peptides in the fractions collected from the first RPLC separation. The phosphoproteome coverage was further improved by using two types of instruments, i.e. TripleTOF 5600 and LTQ Orbitrap Velos. A total of 22,446 phosphorylation sites, corresponding to 6526 nonredundant phosphoproteins were finally identified from normal human liver tissues. Of these sites, 15,229 sites were confidently localized with Ascore >= 13. This dataset was the largest phosphoproteome dataset of human liver. It can be a public resource for the liver research community and holds promise for further biology studies.
Language英语
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
URL查看原文
WOS IDWOS:000331916300020
Citation statistics
Cited Times:103[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119681
Collection中国科学院大连化学物理研究所
Corresponding AuthorYe ML(叶明亮); Zou HF(邹汉法)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China
Recommended Citation
GB/T 7714
Bian, Yangyang,Song, Chunxia,Cheng, Kai,et al. An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome[J]. JOURNAL OF PROTEOMICS,2014,96:253-262.
APA Bian, Yangyang.,Song, Chunxia.,Cheng, Kai.,Dong, Mingming.,Wang, Fangjun.,...&邹汉法.(2014).An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.JOURNAL OF PROTEOMICS,96,253-262.
MLA Bian, Yangyang,et al."An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome".JOURNAL OF PROTEOMICS 96(2014):253-262.
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