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学科主题: 物理化学
题名: A simple microfluidic strategy for cell migration assay in an in vitro wound-healing model
作者: Zhang, Min1;  Li, Hongjing2;  Ma, Huipeng3;  Qin, Jianhua1
通讯作者: 秦建华
刊名: WOUND REPAIR AND REGENERATION
发表日期: 2013-11-01
DOI: 10.1111/wrr.12106
卷: 21, 期:6, 页:897-903
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 待补充
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Cell Biology ;  Dermatology ;  Medicine, Research & Experimental ;  Surgery
资助者: 1,1
研究领域[WOS]: Cell Biology ;  Dermatology ;  Research & Experimental Medicine ;  Surgery
英文摘要: In vitro scratch wound assays are commonly used strategies to measure cell repair rate, facilitating the study of cell migration, tissue reorganization, and cell division. This work presented a simple and novel microfluidic device that allowed a quantitative investigation of the cell migration and cell proliferation behaviors in an in vitro wound-healing model, especially focused on the scratch assay. The microfluidic device is composed of four units, which include cell growth regions and cell-free regions created by micropillars. Using this device, we evaluated the proliferation and migration process of human gastric epithelial cells in the presence of different concentrations of the epidermal growth factor, and investigated the migration behavior of mesenchymal stem cells toward tumor cells as well. This approach has the unique capability to create localized cell-free regions in parallel, and facilitate quantitative research on cell migration in the wound-healing process, providing a powerful platform for elucidating the mechanism of cell migration in regeneration medicine.
关键词[WOS]: MOTILITY ;  COMPLEX
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000326327100162
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119682
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 1, Dept Orthoped, Dalian, Liaoning, Peoples R China
3.Dalian Med Univ, Coll Med Lab, Dalian, Liaoning, Peoples R China

Recommended Citation:
Zhang, Min,Li, Hongjing,Ma, Huipeng,et al. A simple microfluidic strategy for cell migration assay in an in vitro wound-healing model[J]. WOUND REPAIR AND REGENERATION,2013,21(6):897-903.
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