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学科主题: 物理化学
题名: One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides
作者: Fang, Chunli1;  Xiong, Zhichao2;  Qin, Hongqiang3;  Huang, Guang3;  Liu, Jing3;  Ye, Mingliang3;  Feng, Shun1;  Zou, Hanfa3
通讯作者: 封顺 ;  邹汉法
关键词: Glycopeptides ;  Magnetic colloidal nanocrystal clusters ;  Chitosan ;  Hydrophilic interaction liquid ;  chromatography
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2014-09-02
DOI: 10.1016/j.aca.2014.05.037
卷: 841, 页:99-105
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 待补充
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
资助者: 3,3
研究领域[WOS]: Chemistry
英文摘要: Selective enrichment of glycopeptides prior to the mass spectrometry (MS) analysis is essential due to ion suppression effect during ionization caused by the co-presence of non-glycosylated peptides. Among the enrichment approaches, hydrophilic interaction liquid chromatography (HILIC) based on magnetic separation has become a popular method in recent years. As the conventional synthesis procedures of these materials are tedious and time-consuming with at least four steps. Herein, magnetic colloidal nanocrystal clusters coated with chitosan (Fe3O4@CS MCNCs) have been successfully prepared by a simple one-pot method. The resulting Fe3O4@CS MCNCs demonstrated an excellent ability for glycopeptide enrichment with high selectivity, low detection limit and high binding capacity. Furthermore, in the analysis of real complicated biological sample, 283 unique N-glycosylation sites corresponding to 175 glycosylated proteins were identified in three replicate analyses of 45 mu g protein sample extracted from HeLa cells, indicating the great potential in detection and identification of low abundant glycopeptides in glycoproteome analysis. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: INTERACTION LIQUID-CHROMATOGRAPHY ;  MASS-SPECTROMETRY ;  GLYCOSYLATION ;  NANOPARTICLES ;  POLYSACCHARIDE ;  IDENTIFICATION ;  SILICA
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000339990900013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119694
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Xinjiang Univ, Coll Chem & Chem Engn, Minist Educ Xinjiang Uyghur Autonomous Reg, Key Lab Oil Gas Fine Chem, Urumqi 830046, Peoples R China
2.E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Fang, Chunli,Xiong, Zhichao,Qin, Hongqiang,et al. One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides[J]. ANALYTICA CHIMICA ACTA,2014,841:99-105.
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