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学科主题: 物理化学
题名: Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip
作者: Wen, Hui1;  Gao, Xinghua1;  Qin, Jianhua1
通讯作者: 秦建华
刊名: INTEGRATIVE BIOLOGY
发表日期: 2014
DOI: 10.1039/c3ib40191j
卷: 6, 期:1, 页:35-43
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 待补充
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Cell Biology
资助者: 1,1
研究领域[WOS]: Cell Biology
英文摘要: C. elegans is widely used as a model organism in the study of aging and evaluation of anti-aging drugs due to its unique characteristics. In this work, we set out to investigate polydatin, a natural resveratrol glycoside, and its role in extending lifespan, improving oxidative stress resistance, and the possible regulation mechanism involved in the Insulin/IGF-1 signaling (IIS) pathway for the first time by using a flexible microfluidic device. The effects of polydatin on the lifespan, oxidative stress resistance, mobility and the expression of aging-related proteins and genes were explored. Polydatin was found to significantly extend the mean lifespan of worms by up to 30.7% and 62.1% under normal and acute stress conditions respectively. It improved the expression of the inducible oxidative stress protein (GST-4) and corresponding stroke frequencies in the transgenic CL2166 strain. Moreover, it also increased SOD-3::GFP expression in CF1553 worms and promoted DAF-16 nucleus translocation in TJ356 worms. The longevity-extending role of polydatin is partly attributed to its anti-oxidative activity and increased oxidative stress resistance by regulating the stress-resistance related proteins SOD-3, and daf-16 expression at protein and mRNA levels involved in the IIS pathway. The established microfluidic platform is capable of flexible operation with multiple functions, which not only supports the individual worm's long-term culture with sufficient nutrient exchange, but also facilitates mobility monitoring of the worm, immobilizing and imaging in a controllable and parallel manner. These interesting findings reported here highlight the significance of the natural compound polydatin in the study of aging-related diseases, and the utility of the microfluidic platform for applications in aging studies.
关键词[WOS]: LIFE-SPAN ;  C-ELEGANS ;  RESVERATROL ;  ACTIVATION ;  DISEASES ;  PROTEIN ;  STRESS ;  INJURY ;  PICEID ;  GENES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000328885900004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119696
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Biotechnol Lab, Dalian 116023, Peoples R China

Recommended Citation:
Wen, Hui,Gao, Xinghua,Qin, Jianhua. Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip[J]. INTEGRATIVE BIOLOGY,2014,6(1):35-43.
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