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学科主题物理化学
Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip
Wen, Hui; Gao, Xinghua; Qin, Jianhua; Qin JH(秦建华)
刊名INTEGRATIVE BIOLOGY
2014
ISSN1757-9694
DOI10.1039/c3ib40191j
6期:1页:35-43
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1807
产权排名待补充
WOS标题词Science & Technology ; Life Sciences & Biomedicine
资助者1,1 ; 1,1 ; 1,1 ; 1,1
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]LIFE-SPAN ; C-ELEGANS ; RESVERATROL ; ACTIVATION ; DISEASES ; PROTEIN ; STRESS ; INJURY ; PICEID ; GENES
英文摘要C. elegans is widely used as a model organism in the study of aging and evaluation of anti-aging drugs due to its unique characteristics. In this work, we set out to investigate polydatin, a natural resveratrol glycoside, and its role in extending lifespan, improving oxidative stress resistance, and the possible regulation mechanism involved in the Insulin/IGF-1 signaling (IIS) pathway for the first time by using a flexible microfluidic device. The effects of polydatin on the lifespan, oxidative stress resistance, mobility and the expression of aging-related proteins and genes were explored. Polydatin was found to significantly extend the mean lifespan of worms by up to 30.7% and 62.1% under normal and acute stress conditions respectively. It improved the expression of the inducible oxidative stress protein (GST-4) and corresponding stroke frequencies in the transgenic CL2166 strain. Moreover, it also increased SOD-3::GFP expression in CF1553 worms and promoted DAF-16 nucleus translocation in TJ356 worms. The longevity-extending role of polydatin is partly attributed to its anti-oxidative activity and increased oxidative stress resistance by regulating the stress-resistance related proteins SOD-3, and daf-16 expression at protein and mRNA levels involved in the IIS pathway. The established microfluidic platform is capable of flexible operation with multiple functions, which not only supports the individual worm's long-term culture with sufficient nutrient exchange, but also facilitates mobility monitoring of the worm, immobilizing and imaging in a controllable and parallel manner. These interesting findings reported here highlight the significance of the natural compound polydatin in the study of aging-related diseases, and the utility of the microfluidic platform for applications in aging studies.
语种英语
资助者1,1 ; 1,1 ; 1,1 ; 1,1
原文出处查看原文
WOS记录号WOS:000328885900004
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119696
专题中国科学院大连化学物理研究所
通讯作者Qin JH(秦建华)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Biotechnol Lab, Dalian 116023, Peoples R China
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GB/T 7714
Wen, Hui,Gao, Xinghua,Qin, Jianhua,et al. Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip[J]. INTEGRATIVE BIOLOGY,2014,6(1):35-43.
APA Wen, Hui,Gao, Xinghua,Qin, Jianhua,&秦建华.(2014).Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip.INTEGRATIVE BIOLOGY,6(1),35-43.
MLA Wen, Hui,et al."Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip".INTEGRATIVE BIOLOGY 6.1(2014):35-43.
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