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学科主题: 物理化学
题名: Analysis of the human urine endogenous peptides by nanoparticle extraction and mass spectrometry identification
作者: Yang, Xiaomin2;  Hu, Lianghai1, 3;  Ye, Mingliang3;  Zou, Hanfa3
通讯作者: 胡良海
关键词: Proteomics ;  Peptidomics ;  Human urine ;  Nanoparticle ;  Two-dimensional ;  Biomarker
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2014-06-04
DOI: 10.1016/j.aca.2014.04.040
卷: 829, 页:40-47
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 待补充
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
资助者: 3,2
研究领域[WOS]: Chemistry
英文摘要: Peptides in urine are excreted by kidney from the blood and tissues, which are composed of a large amount of hormones, cytokines, regulatory factors and the metabolized fragments of proteins. The peptide distribution in urine will reflect the physiological and pathophysiological processes in body. In past, limited information was reported about the composition of the peptides in urine. One possible reason is that the peptides in urine are fairly low abundant and there are high concentrations of salts and organic metabolites in the urine. In this report, we extracted the peptides from human urine by highly ordered mesoporous silica particles with the pore size of 2 nm, which will exclude the high molecular weight proteins over 12 kDa. The extracted peptides were then separated into fractions according to their molecular weight by size exclusion chromatography. Each of the fractions was further analyzed by MALDI-TOF MS and mu RPLC-MS/MS. Totally, 193 peptides were identified by two-dimensional SEC/mu RPLC-MS/MS analysis. By analyzing the progenitor protein of the peptides; we found that two-thirds of the proteins differed from the reported urine proteome database, and the high abundant proteins in urine proteome were less detected in the urine peptidome. The developed extraction and separation methods were efficient for the profiling of the endogenous peptides in human urine. The peptidome in human urine was complementary to the human urinary proteome and may provide an emerging field for biomarker discovery. Published by Elsevier B.V.
关键词[WOS]: HUMAN PLASMA ;  BIOMARKER DISCOVERY ;  SERUM PEPTIDOME ;  COMPREHENSIVE ANALYSIS ;  LIQUID-CHROMATOGRAPHY ;  SELECTIVE EXTRACTION ;  PROTEIN-FRAGMENTS ;  MESOPOROUS SILICA ;  LC-MS/MS ;  PROTEOMICS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000336388700007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119704
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Jilin Univ, Sch Life Sci, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130012, Jilin, Peoples R China
2.Jilin Univ, Coll Chem, Changchun 130012, Jilin, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Yang, Xiaomin,Hu, Lianghai,Ye, Mingliang,et al. Analysis of the human urine endogenous peptides by nanoparticle extraction and mass spectrometry identification[J]. ANALYTICA CHIMICA ACTA,2014,829:40-47.
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