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学科主题物理化学
Analysis of the human urine endogenous peptides by nanoparticle extraction and mass spectrometry identification
Yang, Xiaomin2; Hu, Lianghai1,3; Ye, Mingliang3; Zou, Hanfa3; Hu LH(胡良海)
关键词Proteomics Peptidomics Human Urine Nanoparticle Two-dimensional Biomarker
刊名ANALYTICA CHIMICA ACTA
2014-06-04
ISSN0003-2670
DOI10.1016/j.aca.2014.04.040
829页:40-47
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1809
产权排名待补充
WOS标题词Science & Technology ; Physical Sciences
资助者3,2 ; 3,2 ; 3,2 ; 3,2
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]HUMAN PLASMA ; BIOMARKER DISCOVERY ; SERUM PEPTIDOME ; COMPREHENSIVE ANALYSIS ; LIQUID-CHROMATOGRAPHY ; SELECTIVE EXTRACTION ; PROTEIN-FRAGMENTS ; MESOPOROUS SILICA ; LC-MS/MS ; PROTEOMICS
英文摘要Peptides in urine are excreted by kidney from the blood and tissues, which are composed of a large amount of hormones, cytokines, regulatory factors and the metabolized fragments of proteins. The peptide distribution in urine will reflect the physiological and pathophysiological processes in body. In past, limited information was reported about the composition of the peptides in urine. One possible reason is that the peptides in urine are fairly low abundant and there are high concentrations of salts and organic metabolites in the urine. In this report, we extracted the peptides from human urine by highly ordered mesoporous silica particles with the pore size of 2 nm, which will exclude the high molecular weight proteins over 12 kDa. The extracted peptides were then separated into fractions according to their molecular weight by size exclusion chromatography. Each of the fractions was further analyzed by MALDI-TOF MS and mu RPLC-MS/MS. Totally, 193 peptides were identified by two-dimensional SEC/mu RPLC-MS/MS analysis. By analyzing the progenitor protein of the peptides; we found that two-thirds of the proteins differed from the reported urine proteome database, and the high abundant proteins in urine proteome were less detected in the urine peptidome. The developed extraction and separation methods were efficient for the profiling of the endogenous peptides in human urine. The peptidome in human urine was complementary to the human urinary proteome and may provide an emerging field for biomarker discovery. Published by Elsevier B.V.
语种英语
资助者3,2 ; 3,2 ; 3,2 ; 3,2
原文出处查看原文
WOS记录号WOS:000336388700007
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119704
专题中国科学院大连化学物理研究所
通讯作者Hu LH(胡良海)
作者单位1.Jilin Univ, Sch Life Sci, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130012, Jilin, Peoples R China
2.Jilin Univ, Coll Chem, Changchun 130012, Jilin, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Yang, Xiaomin,Hu, Lianghai,Ye, Mingliang,et al. Analysis of the human urine endogenous peptides by nanoparticle extraction and mass spectrometry identification[J]. ANALYTICA CHIMICA ACTA,2014,829:40-47.
APA Yang, Xiaomin,Hu, Lianghai,Ye, Mingliang,Zou, Hanfa,&胡良海.(2014).Analysis of the human urine endogenous peptides by nanoparticle extraction and mass spectrometry identification.ANALYTICA CHIMICA ACTA,829,40-47.
MLA Yang, Xiaomin,et al."Analysis of the human urine endogenous peptides by nanoparticle extraction and mass spectrometry identification".ANALYTICA CHIMICA ACTA 829(2014):40-47.
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