DICP OpenIR
Subject Area物理化学
Analysis of the human urine endogenous peptides by nanoparticle extraction and mass spectrometry identification
Yang, Xiaomin2; Hu, Lianghai1,3; Ye, Mingliang3; Zou, Hanfa3; Hu LH(胡良海)
KeywordProteomics Peptidomics Human Urine Nanoparticle Two-dimensional Biomarker
Source PublicationANALYTICA CHIMICA ACTA
2014-06-04
ISSN0003-2670
DOI10.1016/j.aca.2014.04.040
Volume829Pages:40-47
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1809
Contribution Rank待补充
WOS HeadingsScience & Technology ; Physical Sciences
Funding Organization3,2 ; 3,2 ; 3,2 ; 3,2
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordHUMAN PLASMA ; BIOMARKER DISCOVERY ; SERUM PEPTIDOME ; COMPREHENSIVE ANALYSIS ; LIQUID-CHROMATOGRAPHY ; SELECTIVE EXTRACTION ; PROTEIN-FRAGMENTS ; MESOPOROUS SILICA ; LC-MS/MS ; PROTEOMICS
AbstractPeptides in urine are excreted by kidney from the blood and tissues, which are composed of a large amount of hormones, cytokines, regulatory factors and the metabolized fragments of proteins. The peptide distribution in urine will reflect the physiological and pathophysiological processes in body. In past, limited information was reported about the composition of the peptides in urine. One possible reason is that the peptides in urine are fairly low abundant and there are high concentrations of salts and organic metabolites in the urine. In this report, we extracted the peptides from human urine by highly ordered mesoporous silica particles with the pore size of 2 nm, which will exclude the high molecular weight proteins over 12 kDa. The extracted peptides were then separated into fractions according to their molecular weight by size exclusion chromatography. Each of the fractions was further analyzed by MALDI-TOF MS and mu RPLC-MS/MS. Totally, 193 peptides were identified by two-dimensional SEC/mu RPLC-MS/MS analysis. By analyzing the progenitor protein of the peptides; we found that two-thirds of the proteins differed from the reported urine proteome database, and the high abundant proteins in urine proteome were less detected in the urine peptidome. The developed extraction and separation methods were efficient for the profiling of the endogenous peptides in human urine. The peptidome in human urine was complementary to the human urinary proteome and may provide an emerging field for biomarker discovery. Published by Elsevier B.V.
Language英语
Funding Organization3,2 ; 3,2 ; 3,2 ; 3,2
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WOS IDWOS:000336388700007
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119704
Collection中国科学院大连化学物理研究所
Corresponding AuthorHu LH(胡良海)
Affiliation1.Jilin Univ, Sch Life Sci, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130012, Jilin, Peoples R China
2.Jilin Univ, Coll Chem, Changchun 130012, Jilin, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Yang, Xiaomin,Hu, Lianghai,Ye, Mingliang,et al. Analysis of the human urine endogenous peptides by nanoparticle extraction and mass spectrometry identification[J]. ANALYTICA CHIMICA ACTA,2014,829:40-47.
APA Yang, Xiaomin,Hu, Lianghai,Ye, Mingliang,Zou, Hanfa,&胡良海.(2014).Analysis of the human urine endogenous peptides by nanoparticle extraction and mass spectrometry identification.ANALYTICA CHIMICA ACTA,829,40-47.
MLA Yang, Xiaomin,et al."Analysis of the human urine endogenous peptides by nanoparticle extraction and mass spectrometry identification".ANALYTICA CHIMICA ACTA 829(2014):40-47.
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