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学科主题物理化学
The on-bead digestion of protein corona on nanoparticles by trypsin immobilized on the magnetic nanoparticle
Hu, Zhengyan1,2; Zhao, Liang1,2; Zhang, Hongyan1,2; Zhang, Yi1,2; Wu, Ren'an1; Zou, Hanfa1; Wu RA(吴仁安); Zou HF(邹汉法)
关键词Protein Corona Digestion Immobilized Trypsin Magnetic Nanoparticle Fe3o4 Plasma
刊名JOURNAL OF CHROMATOGRAPHY A
2014-03-21
ISSN0021-9673
DOI10.1016/j.chroma.2014.01.077
1334页:55-63
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1809
产权排名待补充
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
资助者1,1 ; 1,1 ; 1,1 ; 1,1
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]COPOLYMER NANOPARTICLES ; SURFACE-PROPERTIES ; NANOMATERIALS ; SIZE ; MICROSPHERES ; ADSORPTION ; CELLS ; OXIDE
英文摘要Proteins interacting with nanoparticles would form the protein coronas on the surface of nanoparticles in biological systems, which would critically impact the biological identities of nanoparticles and/or result in the physiological and pathological consequences. The enzymatic digestion of protein corona was the primary step to achieve the identification of protein components of the protein corona for the bottomup proteomic approaches. In this study, the investigation on the tryptic digestion of protein corona by the immobilized trypsin on a magnetic nanoparticle was carried out for the first time. As a comparison with the usual overnight long-time digestion and the severe self-digestion of free trypsin, the on-bead digestion of protein corona by the immobilized trypsin could be accomplished within 1 h, along with the significantly reduced self-digestion of trypsin and the improved reproducibility on the identification of proteins by the mass spectrometry-based proteomic approach. It showed that the number of identified bovine serum (BS) proteins on the commercial Fe3O4 nanoparticles was increased by 13% for the immobilized trypsin with 1 h digestion as compared to that of using free trypsin with even overnight digestion. In addition, the on-bead digestion of using the immobilized trypsin was further applied on the identification of human plasma protein corona on the commercial Fe3O4 nanoparticles, which leads the efficient digestion of the human plasma proteins and the identification of 149 human plasma proteins corresponding to putative critical pathways and biological processes. (c) 2014 Elsevier B.V. All rights reserved.
语种英语
资助者1,1 ; 1,1 ; 1,1 ; 1,1
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WOS记录号WOS:000333491000007
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119707
专题中国科学院大连化学物理研究所
通讯作者Wu RA(吴仁安); Zou HF(邹汉法)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
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Hu, Zhengyan,Zhao, Liang,Zhang, Hongyan,et al. The on-bead digestion of protein corona on nanoparticles by trypsin immobilized on the magnetic nanoparticle[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1334:55-63.
APA Hu, Zhengyan.,Zhao, Liang.,Zhang, Hongyan.,Zhang, Yi.,Wu, Ren'an.,...&邹汉法.(2014).The on-bead digestion of protein corona on nanoparticles by trypsin immobilized on the magnetic nanoparticle.JOURNAL OF CHROMATOGRAPHY A,1334,55-63.
MLA Hu, Zhengyan,et al."The on-bead digestion of protein corona on nanoparticles by trypsin immobilized on the magnetic nanoparticle".JOURNAL OF CHROMATOGRAPHY A 1334(2014):55-63.
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