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学科主题: 物理化学
题名: The on-bead digestion of protein corona on nanoparticles by trypsin immobilized on the magnetic nanoparticle
作者: Hu, Zhengyan1, 2;  Zhao, Liang1, 2;  Zhang, Hongyan1, 2;  Zhang, Yi1, 2;  Wu, Ren'an1;  Zou, Hanfa1
通讯作者: 吴仁安 ;  邹汉法
关键词: Protein corona ;  Digestion ;  Immobilized trypsin ;  Magnetic nanoparticle ;  Fe3O4 ;  Plasma
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2014-03-21
DOI: 10.1016/j.chroma.2014.01.077
卷: 1334, 页:55-63
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 待补充
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
资助者: 1,1
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Proteins interacting with nanoparticles would form the protein coronas on the surface of nanoparticles in biological systems, which would critically impact the biological identities of nanoparticles and/or result in the physiological and pathological consequences. The enzymatic digestion of protein corona was the primary step to achieve the identification of protein components of the protein corona for the bottomup proteomic approaches. In this study, the investigation on the tryptic digestion of protein corona by the immobilized trypsin on a magnetic nanoparticle was carried out for the first time. As a comparison with the usual overnight long-time digestion and the severe self-digestion of free trypsin, the on-bead digestion of protein corona by the immobilized trypsin could be accomplished within 1 h, along with the significantly reduced self-digestion of trypsin and the improved reproducibility on the identification of proteins by the mass spectrometry-based proteomic approach. It showed that the number of identified bovine serum (BS) proteins on the commercial Fe3O4 nanoparticles was increased by 13% for the immobilized trypsin with 1 h digestion as compared to that of using free trypsin with even overnight digestion. In addition, the on-bead digestion of using the immobilized trypsin was further applied on the identification of human plasma protein corona on the commercial Fe3O4 nanoparticles, which leads the efficient digestion of the human plasma proteins and the identification of 149 human plasma proteins corresponding to putative critical pathways and biological processes. (c) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: COPOLYMER NANOPARTICLES ;  SURFACE-PROPERTIES ;  NANOMATERIALS ;  SIZE ;  MICROSPHERES ;  ADSORPTION ;  CELLS ;  OXIDE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000333491000007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119707
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Hu, Zhengyan,Zhao, Liang,Zhang, Hongyan,et al. The on-bead digestion of protein corona on nanoparticles by trypsin immobilized on the magnetic nanoparticle[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1334:55-63.
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