DICP OpenIR
Subject Area物理化学
The on-bead digestion of protein corona on nanoparticles by trypsin immobilized on the magnetic nanoparticle
Hu, Zhengyan1,2; Zhao, Liang1,2; Zhang, Hongyan1,2; Zhang, Yi1,2; Wu, Ren'an1; Zou, Hanfa1; Wu RA(吴仁安); Zou HF(邹汉法)
KeywordProtein Corona Digestion Immobilized Trypsin Magnetic Nanoparticle Fe3o4 Plasma
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2014-03-21
ISSN0021-9673
DOI10.1016/j.chroma.2014.01.077
Volume1334Pages:55-63
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1809
Contribution Rank待补充
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordCOPOLYMER NANOPARTICLES ; SURFACE-PROPERTIES ; NANOMATERIALS ; SIZE ; MICROSPHERES ; ADSORPTION ; CELLS ; OXIDE
AbstractProteins interacting with nanoparticles would form the protein coronas on the surface of nanoparticles in biological systems, which would critically impact the biological identities of nanoparticles and/or result in the physiological and pathological consequences. The enzymatic digestion of protein corona was the primary step to achieve the identification of protein components of the protein corona for the bottomup proteomic approaches. In this study, the investigation on the tryptic digestion of protein corona by the immobilized trypsin on a magnetic nanoparticle was carried out for the first time. As a comparison with the usual overnight long-time digestion and the severe self-digestion of free trypsin, the on-bead digestion of protein corona by the immobilized trypsin could be accomplished within 1 h, along with the significantly reduced self-digestion of trypsin and the improved reproducibility on the identification of proteins by the mass spectrometry-based proteomic approach. It showed that the number of identified bovine serum (BS) proteins on the commercial Fe3O4 nanoparticles was increased by 13% for the immobilized trypsin with 1 h digestion as compared to that of using free trypsin with even overnight digestion. In addition, the on-bead digestion of using the immobilized trypsin was further applied on the identification of human plasma protein corona on the commercial Fe3O4 nanoparticles, which leads the efficient digestion of the human plasma proteins and the identification of 149 human plasma proteins corresponding to putative critical pathways and biological processes. (c) 2014 Elsevier B.V. All rights reserved.
Language英语
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
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WOS IDWOS:000333491000007
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119707
Collection中国科学院大连化学物理研究所
Corresponding AuthorWu RA(吴仁安); Zou HF(邹汉法)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Hu, Zhengyan,Zhao, Liang,Zhang, Hongyan,et al. The on-bead digestion of protein corona on nanoparticles by trypsin immobilized on the magnetic nanoparticle[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1334:55-63.
APA Hu, Zhengyan.,Zhao, Liang.,Zhang, Hongyan.,Zhang, Yi.,Wu, Ren'an.,...&邹汉法.(2014).The on-bead digestion of protein corona on nanoparticles by trypsin immobilized on the magnetic nanoparticle.JOURNAL OF CHROMATOGRAPHY A,1334,55-63.
MLA Hu, Zhengyan,et al."The on-bead digestion of protein corona on nanoparticles by trypsin immobilized on the magnetic nanoparticle".JOURNAL OF CHROMATOGRAPHY A 1334(2014):55-63.
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