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学科主题: 物理化学
题名: Highly selective separation of aminoglycoside antibiotics on a zwitterionic Click TE-Cys column
作者: Wei, Jie1;  Shen, Aijin2;  Wan, Huihui3;  Yan, Jingyu2;  Yang, Bingcheng1;  Guo, Zhimou2;  Zhang, Feifang1;  Liang, Xinmiao1, 2
通讯作者: 郭志谋
关键词: Aminoglycosides ;  Hydrophilic interaction chromatography ;  Separation selectivity ;  Zwitterionic stationary phases
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2014-07-01
DOI: 10.1002/jssc.201400080
卷: 37, 期:14, 页:1781-1787
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 待补充
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
资助者: 2,2
研究领域[WOS]: Chemistry
英文摘要: Hydrophilic interaction liquid chromatography has emerged as a valuable alternative approach to ion-pair chromatography for the separation of aminoglycoside antibiotics in recent years. However, the resolution of structurally related aminoglycosides is a great challenge owing to the limited selectivity. In this work, a cysteine-based zwitterionic stationary phase (named Click TE-Cys) was utilized and compared with five commonly used hydrophilic interaction liquid chromatography columns. Click TE-Cys displayed much better selectivity for structurally similar aminoglycosides. The retention behaviors of aminoglycosides were investigated in detail, revealing that low pH (2.7 or 3.0) and high buffer concentration (>= 50 mM) were preferable for achieving good peak shape and selectivity. Effective resolution of ten aminoglycosides including spectinomycin, dihydrostreptomycin, streptomycin, gentamicin C1, gentamicin C2/C2a, gentamicin C1a, kanamycin, paromonycin, tobramycin, and neomycin was realized at optimized conditions. Additionally, spectinomycin and its related impurities were successfully resolved. The results indicated the great potential of the Click TE-Cys column in the separation of aminoglycoside mixtures and related impurities.
关键词[WOS]: TANDEM-MASS-SPECTROMETRY ;  HYDROPHILIC INTERACTION CHROMATOGRAPHY ;  PERFORMANCE LIQUID-CHROMATOGRAPHY ;  LIGHT-SCATTERING DETECTION ;  STATIONARY-PHASE ;  CONFIRMATORY METHOD ;  HPLC-ELSD ;  RESIDUES ;  DIHYDROSTREPTOMYCIN ;  STREPTOMYCIN
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000339669700009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119711
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
3.Dalian Univ Technol, Chem Anal & Res Ctr, Fac Chem Environm & Biol Sci & Technol, Dalian, Peoples R China

Recommended Citation:
Wei, Jie,Shen, Aijin,Wan, Huihui,et al. Highly selective separation of aminoglycoside antibiotics on a zwitterionic Click TE-Cys column[J]. JOURNAL OF SEPARATION SCIENCE,2014,37(14):1781-1787.
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