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学科主题: 物理化学
题名: Synthesis of zwitterionic polymer brushes hybrid silica nanoparticles via controlled polymerization for highly efficient enrichment of glycopeptides
作者: Huang, Guang1, 2, 3;  Xiong, Zhichao1, 4;  Qin, Hongqiang1;  Zhu, Jun1, 2;  Sun, Zhen1, 2;  Zhang, Yi1, 2;  Peng, Xiaojun3;  Ou, Junjie1;  Zou, Hanfa1
通讯作者: 欧俊杰 ;  邹汉法
关键词: Controlled polymerization ;  Hydrophilic interaction chromatography ;  Silica nanoparticles ;  Glycopeptide enrichment ;  Glycoproteomics
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2014-01-27
DOI: 10.1016/j.aca.2013.11.049
卷: 809, 页:61-68
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 待补充
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
资助者: 1,1
研究领域[WOS]: Chemistry
英文摘要: Zwitterionic hydrophilic interaction chromatography (ZIC-HILIC) materials have been increasingly attractive in glycopeptide enrichment. However, the traditional ZIC-HILIC materials are modified with monolayer zwitterionic molecules on the surface, therefore, the hydrophilicity, detection sensitivity and loading capacity are limited. In this work, we synthesized novel silica nanoparticles with uniform poly(2( methacryloyloxy) ethyl) dimethyl-(3-sul-fopropyl) ammonium hydroxide (PMSA) brushes grafted onto the surface via reversible addition-fragmentation chain transfer (RAFT) polymerization (denoted as SiO2-RAFT@ PMSA). The resulting SiO2-RAFT@ PMSA nanoparticles demonstrated low detection limit (10 fmol) and high recovery yield (over 88%) for glycopeptide enrichment from tryptic digest of human IgG. The SiO2-RAFT@ PMSA nanoparticles were further applied for the analysis of mouse liver glycoproteome, a total number of 303 unique N-glycosylation sites corresponding to 185 glycoproteins was reliably profiled in three replicate nano-LC-MS/MS runs. Significantly, more glycopeptides were identified than those of nanoparticles, monolayer MSA molecules modified SiO2@single-MSA and nonuniform multilayer PMSA brushes coated SiO2@PMSA, as well as commercial ZIC@HILIC beads and Click Maltose beads. The excellent performance of SiO2-RAFT@PMSA nanoparticles results from the non-fouling property, a large quantity of functional molecules and suitable link arms provided by uniform PMSA brushes, as well as efficient interaction between glycopeptides and uniform PMSA brushes. It is concluded that the synthesized SiO2-RAFT@PMSA nanoparticles exhibit great potential in glycoproteome analysis. Moreover, this strategy to modify nanopaticles with uniform polymer brushes via RAFT polymerization can also be explored to design other types of materials for bioseparation application. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.
关键词[WOS]: HYDROPHILIC INTERACTION CHROMATOGRAPHY ;  INTERACTION LIQUID-CHROMATOGRAPHY ;  FRAGMENTATION CHAIN TRANSFER ;  N-GLYCOSYLATED PROTEINS ;  MASS-SPECTROMETRY ;  RADICAL POLYMERIZATION ;  SELECTIVE ENRICHMENT ;  FACILE SYNTHESIS ;  LINKED GLYCOPEPTIDES ;  ACID
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000329580500008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119713
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
4.E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 20023, Peoples R China

Recommended Citation:
Huang, Guang,Xiong, Zhichao,Qin, Hongqiang,et al. Synthesis of zwitterionic polymer brushes hybrid silica nanoparticles via controlled polymerization for highly efficient enrichment of glycopeptides[J]. ANALYTICA CHIMICA ACTA,2014,809:61-68.
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