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学科主题: 物理化学
题名: Efficient enrichment of glycopeptides using phenylboronic acid polymer brush modified silica microspheres
作者: Li, Xiuling1;  Liu, Hongliang2;  Qing, Guangyan3;  Wang, Shutao2;  Liang, Xinmiao1
通讯作者: 梁鑫淼
刊名: JOURNAL OF MATERIALS CHEMISTRY B
发表日期: 2014
DOI: 10.1039/c4tb00130c
卷: 2, 期:16, 页:2276-2281
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 待补充
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Materials Science, Biomaterials
资助者: 1,1
研究领域[WOS]: Materials Science
英文摘要: For the development of rapid glycopeptide enrichment materials, conventional monolayer phenylboronic acid (PBA) based materials inevitably encounter many problems, such as low loading efficiency, long incubation time, and unsatisfactory selectivity. Extending the materials from a 1D monolayer to a 3D polymeric matrix will be one of the best candidates tackling these problems. In this work, a PBA-based polymer material (denoted as polyPBA@SiO2) was developed, in which flexible PBA polymer brushes were immobilized on the surface of silica microspheres, constructing an ideal platform for the efficient enrichment of glycopeptides. This material exhibits stronger interaction with glycopeptides in a higher concentration of organic solvent than in aqueous solution, resulting in the high binding capacity of 60 mg g (1). Moreover, higher selectivity for glycopeptides can be achieved with polyPBA@SiO2 than with both monolayer PBA modified silica and commercial PBA-agarose. These unique features of polyPBA@SiO2 could be attributed to the synergistic effect of polyvalent interactions provided by the polymer brush, specific interaction between PBA and glycopeptides and suppression of the non-specific binding of non-glycopeptides under high ACN concentration.
关键词[WOS]: HYDROPHILIC AFFINITY ISOLATION ;  HIGHLY SPECIFIC ENRICHMENT ;  N-LINKED GLYCOPROTEINS ;  MASS-SPECTROMETRY ;  HYDRAZIDE CHEMISTRY ;  CLICK CHEMISTRY ;  GLYCOSYLATION ;  CHROMATOGRAPHY ;  NANOPARTICLES ;  PROTEINS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000334120800010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119714
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
3.Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, Wuhan 430070, Peoples R China

Recommended Citation:
Li, Xiuling,Liu, Hongliang,Qing, Guangyan,et al. Efficient enrichment of glycopeptides using phenylboronic acid polymer brush modified silica microspheres[J]. JOURNAL OF MATERIALS CHEMISTRY B,2014,2(16):2276-2281.
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