DICP OpenIR
Subject Area物理化学
N-Glycosylation Site Analysis of Proteins from Saccharomyces cerevisiae by Using Hydrophilic Interaction Liquid Chromatography-Based Enrichment, Parallel Deglycosylation, and Mass Spectrometry
Cao, Liwei; Yu, Long; Guo, Zhimou; Shen, Aijin; Guo, Yunu; Liang, Xinmiao; Yu L(于龙); Liang XM(梁鑫淼)
KeywordGlycoproteomics Saccharomyces Cerevisiae Hydrophilic Interaction Liquid Chromatography Glycopeptide Mass Spectrometry Yeast
Source PublicationJOURNAL OF PROTEOME RESEARCH
2014-03-01
ISSN1535-3893
DOI10.1021/pr401049e
Volume13Issue:3Pages:1485-1493
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1803
Contribution Rank待补充
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordCONGENITAL DISORDERS ; LINKED GLYCOPROTEINS ; GLYCOPEPTIDE-ENRICHMENT ; SELECTIVE ENRICHMENT ; SERUM GLYCOPROTEINS ; IDENTIFICATION ; YEAST ; MATRIX ; LECTIN ; QUANTIFICATION
AbstractN-Glycosylation site analysis of baker's yeast Saccharomyces cerevisiae is of fundamental significance to elucidate the molecular mechanism of human congenital disorders of glycosylation (CDG). Here we present a mass spectrometry (MS)-based workflow for the profiling of N-glycosylated sites in S. cerevisiae proteins. In this workflow, proteolytic glycopeptides were enriched by using a hydrophilic material named Click TE-Cys to improve the glycopeptide selectivity and coverage. To enhance the reliability of the identified results, the enriched glycopeptides were subjected to parallel deglycosylation by using two endoglycosidases (i.e., PNGase F and Endo H-f), respectively, prior to LC-MS/MS analysis. On the basis of the workflow, a total of 135 N-glycosylated sites including 6 known, 93 potential, and 36 novel sites were identified and mapped to 79 proteins. Among the novel-type sites, nine sites from eight proteins, which were simultaneously identified via PNGase F and Endo H-f deglycosylation, are believed to possess high confidence. The established workflow, together with the profile of N-glycosylated sites, will contribute to the improvement of S. cerevisiae model for revealing the pathogenesis of CDG.
Language英语
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
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WOS IDWOS:000332756300028
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119718
Collection中国科学院大连化学物理研究所
Corresponding AuthorYu L(于龙); Liang XM(梁鑫淼)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Cao, Liwei,Yu, Long,Guo, Zhimou,et al. N-Glycosylation Site Analysis of Proteins from Saccharomyces cerevisiae by Using Hydrophilic Interaction Liquid Chromatography-Based Enrichment, Parallel Deglycosylation, and Mass Spectrometry[J]. JOURNAL OF PROTEOME RESEARCH,2014,13(3):1485-1493.
APA Cao, Liwei.,Yu, Long.,Guo, Zhimou.,Shen, Aijin.,Guo, Yunu.,...&梁鑫淼.(2014).N-Glycosylation Site Analysis of Proteins from Saccharomyces cerevisiae by Using Hydrophilic Interaction Liquid Chromatography-Based Enrichment, Parallel Deglycosylation, and Mass Spectrometry.JOURNAL OF PROTEOME RESEARCH,13(3),1485-1493.
MLA Cao, Liwei,et al."N-Glycosylation Site Analysis of Proteins from Saccharomyces cerevisiae by Using Hydrophilic Interaction Liquid Chromatography-Based Enrichment, Parallel Deglycosylation, and Mass Spectrometry".JOURNAL OF PROTEOME RESEARCH 13.3(2014):1485-1493.
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