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学科主题: 物理化学
题名: Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins
作者: Sun, Zhen1;  Dong, Jiaqiang2, 3;  Zhang, Song2, 3;  Hu, Zhengyan1;  Cheng, Kai1;  Li, Kai2, 3;  Xu, Bo1;  Ye, Mingliang1;  Nie, Yongzhan2, 3;  Fan, Daiming2, 3;  Zou, Hanfa1
通讯作者: Daiming Fan ;  邹汉法
关键词: quantification ;  glycoprotein ;  chemoresistance ;  leukemia ;  CSC ;  SWATH MS
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2014-03-01
卷: 13, 期:3, 页:1593-1601
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 待补充
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
资助者: 1,1
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Chemoresistance remains the most significant obstacle to successful chemotherapy for leukemia, and its exact mechanism is still unknown. In this work, we used the cell-surface capturing method together with quantitative proteomics to investigate differences in the glycoproteomes of adriamycin-sensitive and adriamycin-resistant leukemia cells. Two quantitative methods, isotopic dimethyl labeling and SWATH, were used to quantify glycoproteins, and 35 glycoproteins were quantified by both methods. High correlation was observed between the glycoproteins quantified by the above two methods, and 15 glycoproteins displayed a consistent significant change trend in both sets of quantitative results. These 15 proteins included classical multidrug resistance-related glycoproteins such as ABCB1 as well as a set of novel glycoproteins that have not previously been reported to be associated with chemoresistance in leukemia cells. Further validation with quantitative real-time PCR and Western blotting confirmed the proteomic screening results. Subsequent functional experiments based on RNA interference technology showed that CTSD, FKBP10, and SLC2A1 are novel genes that participate in the acquisition and maintenance of the adriamycin-resistant phenotype in leukemia cells.
关键词[WOS]: MULTIDRUG-RESISTANCE ;  CATHEPSIN-D ;  MASS-SPECTROMETRY ;  CANCER-CELLS ;  N-GLYCOSYLATION ;  PROTEIN ;  PROTEOMICS ;  EXPRESSION ;  QUANTIFICATION ;  CONTRIBUTES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000332756300038
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119728
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Fourth Mil Med Univ, State Key Lab Canc Biol, Xian 710032, Shaanxi Provinc, Peoples R China
3.Fourth Mil Med Univ, Xijing Hosp Digest Dis, Xian 710032, Shaanxi Provinc, Peoples R China

Recommended Citation:
Sun, Zhen,Dong, Jiaqiang,Zhang, Song,et al. Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins[J]. JOURNAL OF PROTEOME RESEARCH,2014,13(3):1593-1601.
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