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学科主题物理化学
Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins
Sun, Zhen1; Dong, Jiaqiang2,3; Zhang, Song2,3; Hu, Zhengyan1; Cheng, Kai1; Li, Kai2,3; Xu, Bo1; Ye, Mingliang1; Nie, Yongzhan2,3; Fan, Daiming2,3; Zou, Hanfa1; Daiming Fan; Zou HF(邹汉法)
关键词Quantification Glycoprotein Chemoresistance Leukemia Csc Swath Ms
刊名JOURNAL OF PROTEOME RESEARCH
2014-03-01
ISSN1535-3893
13期:3页:1593-1601
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1809
产权排名待补充
WOS标题词Science & Technology ; Life Sciences & Biomedicine
资助者1,1 ; 1,1 ; 1,1 ; 1,1
类目[WOS]Biochemical Research Methods
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]MULTIDRUG-RESISTANCE ; CATHEPSIN-D ; MASS-SPECTROMETRY ; CANCER-CELLS ; N-GLYCOSYLATION ; PROTEIN ; PROTEOMICS ; EXPRESSION ; QUANTIFICATION ; CONTRIBUTES
英文摘要Chemoresistance remains the most significant obstacle to successful chemotherapy for leukemia, and its exact mechanism is still unknown. In this work, we used the cell-surface capturing method together with quantitative proteomics to investigate differences in the glycoproteomes of adriamycin-sensitive and adriamycin-resistant leukemia cells. Two quantitative methods, isotopic dimethyl labeling and SWATH, were used to quantify glycoproteins, and 35 glycoproteins were quantified by both methods. High correlation was observed between the glycoproteins quantified by the above two methods, and 15 glycoproteins displayed a consistent significant change trend in both sets of quantitative results. These 15 proteins included classical multidrug resistance-related glycoproteins such as ABCB1 as well as a set of novel glycoproteins that have not previously been reported to be associated with chemoresistance in leukemia cells. Further validation with quantitative real-time PCR and Western blotting confirmed the proteomic screening results. Subsequent functional experiments based on RNA interference technology showed that CTSD, FKBP10, and SLC2A1 are novel genes that participate in the acquisition and maintenance of the adriamycin-resistant phenotype in leukemia cells.
语种英语
资助者1,1 ; 1,1 ; 1,1 ; 1,1
原文出处查看原文
WOS记录号WOS:000332756300038
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119728
专题中国科学院大连化学物理研究所
通讯作者Daiming Fan; Zou HF(邹汉法)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Fourth Mil Med Univ, State Key Lab Canc Biol, Xian 710032, Shaanxi Provinc, Peoples R China
3.Fourth Mil Med Univ, Xijing Hosp Digest Dis, Xian 710032, Shaanxi Provinc, Peoples R China
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GB/T 7714
Sun, Zhen,Dong, Jiaqiang,Zhang, Song,et al. Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins[J]. JOURNAL OF PROTEOME RESEARCH,2014,13(3):1593-1601.
APA Sun, Zhen.,Dong, Jiaqiang.,Zhang, Song.,Hu, Zhengyan.,Cheng, Kai.,...&邹汉法.(2014).Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins.JOURNAL OF PROTEOME RESEARCH,13(3),1593-1601.
MLA Sun, Zhen,et al."Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins".JOURNAL OF PROTEOME RESEARCH 13.3(2014):1593-1601.
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