DICP OpenIR
Subject Area物理化学
Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins
Sun, Zhen1; Dong, Jiaqiang2,3; Zhang, Song2,3; Hu, Zhengyan1; Cheng, Kai1; Li, Kai2,3; Xu, Bo1; Ye, Mingliang1; Nie, Yongzhan2,3; Fan, Daiming2,3; Zou, Hanfa1; Daiming Fan; Zou HF(邹汉法)
KeywordQuantification Glycoprotein Chemoresistance Leukemia Csc Swath Ms
Source PublicationJOURNAL OF PROTEOME RESEARCH
2014-03-01
ISSN1535-3893
Volume13Issue:3Pages:1593-1601
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1809
Contribution Rank待补充
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordMULTIDRUG-RESISTANCE ; CATHEPSIN-D ; MASS-SPECTROMETRY ; CANCER-CELLS ; N-GLYCOSYLATION ; PROTEIN ; PROTEOMICS ; EXPRESSION ; QUANTIFICATION ; CONTRIBUTES
AbstractChemoresistance remains the most significant obstacle to successful chemotherapy for leukemia, and its exact mechanism is still unknown. In this work, we used the cell-surface capturing method together with quantitative proteomics to investigate differences in the glycoproteomes of adriamycin-sensitive and adriamycin-resistant leukemia cells. Two quantitative methods, isotopic dimethyl labeling and SWATH, were used to quantify glycoproteins, and 35 glycoproteins were quantified by both methods. High correlation was observed between the glycoproteins quantified by the above two methods, and 15 glycoproteins displayed a consistent significant change trend in both sets of quantitative results. These 15 proteins included classical multidrug resistance-related glycoproteins such as ABCB1 as well as a set of novel glycoproteins that have not previously been reported to be associated with chemoresistance in leukemia cells. Further validation with quantitative real-time PCR and Western blotting confirmed the proteomic screening results. Subsequent functional experiments based on RNA interference technology showed that CTSD, FKBP10, and SLC2A1 are novel genes that participate in the acquisition and maintenance of the adriamycin-resistant phenotype in leukemia cells.
Language英语
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
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WOS IDWOS:000332756300038
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119728
Collection中国科学院大连化学物理研究所
Corresponding AuthorDaiming Fan; Zou HF(邹汉法)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Fourth Mil Med Univ, State Key Lab Canc Biol, Xian 710032, Shaanxi Provinc, Peoples R China
3.Fourth Mil Med Univ, Xijing Hosp Digest Dis, Xian 710032, Shaanxi Provinc, Peoples R China
Recommended Citation
GB/T 7714
Sun, Zhen,Dong, Jiaqiang,Zhang, Song,et al. Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins[J]. JOURNAL OF PROTEOME RESEARCH,2014,13(3):1593-1601.
APA Sun, Zhen.,Dong, Jiaqiang.,Zhang, Song.,Hu, Zhengyan.,Cheng, Kai.,...&邹汉法.(2014).Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins.JOURNAL OF PROTEOME RESEARCH,13(3),1593-1601.
MLA Sun, Zhen,et al."Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins".JOURNAL OF PROTEOME RESEARCH 13.3(2014):1593-1601.
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