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学科主题: 物理化学
题名: Enrichment of cancer stem cell-like cells by culture in alginate gel beads
作者: Xu, Xiao-xi1, 2;  Liu, Chang1, 2;  Liu, Yang1;  Yang, Li3;  Li, Nan1, 2;  Guo, Xin1;  Sun, Guang-wei1;  Ma, Xiao-jun1
通讯作者: 孙广炜 ;  马小军
关键词: Alginate gel bead ;  Cancer stem cell ;  Enrichment ;  Three-dimensional culture ;  Hypoxia-inducible factor
刊名: JOURNAL OF BIOTECHNOLOGY
发表日期: 2014-05-10
DOI: 10.1016/j.jbiotec.2014.02.016
卷: 177, 页:1-12
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1802
产权排名: 待补充
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
资助者: 1,1
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: Cancer stem cells (CSCs) are most likely the reason of cancer reoccurrence and metastasis. For further elucidation of the mechanism underlying the characteristics of CSCs, it is necessary to develop efficient culture systems to culture and expand CSCs. In this study, a three-dimensional (3D) culture system based on alginate gel (ALG) beads was reported to enrich CSCs. Two cell lines derived from different histologic origins were encapsulated in ALG beads respectively and the expansion of CSCs was investigated. Compared with two-dimensional (2D) culture, the proportion of cells with CSC-like phenotypes was significantly increased in ALG beads. Expression levels of CSC-related genes were greater in ALG beads than in 2D culture. The increase of CSC proportion after being cultured within ALG beads was further confirmed by enhanced tumorigenicity in vivo. Moreover, increased metastasis ability and higher anti-cancer drug resistance were also observed in 3D-cultured cells. Furthermore, we found that it was hypoxia, through the upregulation of hypoxia-inducible factors (HIFs) that occurred in ALG beads to induce the increasing of CSC proportion. Therefore, ALG bead was an efficient culture system for CSC enrichment, which might provide a useful platform for CSC research and promote the development of new anti-cancer therapies targeting CSCs. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: HEPATOCELLULAR-CARCINOMA CELLS ;  SOLID TUMORS ;  MATRIX METALLOPROTEINASES ;  STEM/PROGENITOR CELLS ;  3-DIMENSIONAL CULTURE ;  COMPOSITE HYDROGELS ;  INITIATING CELLS ;  DRUG-RESISTANCE ;  HYALURONIC-ACID ;  SELF-RENEWAL
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000333811200001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119749
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Med Univ, Hosp 2, Dalian 116023, Peoples R China

Recommended Citation:
Xu, Xiao-xi,Liu, Chang,Liu, Yang,et al. Enrichment of cancer stem cell-like cells by culture in alginate gel beads[J]. JOURNAL OF BIOTECHNOLOGY,2014,177:1-12.
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