DICP OpenIR
Subject Area物理化学
Enrichment of cancer stem cell-like cells by culture in alginate gel beads
Xu, Xiao-xi1,2; Liu, Chang1,2; Liu, Yang1; Yang, Li3; Li, Nan1,2; Guo, Xin1; Sun, Guang-wei1; Ma, Xiao-jun1; Sun GW(孙广炜); Ma XJ(马小军)
KeywordAlginate Gel Bead Cancer Stem Cell Enrichment Three-dimensional Culture Hypoxia-inducible Factor
Source PublicationJOURNAL OF BIOTECHNOLOGY
2014-05-10
DOI10.1016/j.jbiotec.2014.02.016
Volume177Pages:1-12
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1802
Contribution Rank待补充
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
WOS SubjectBiotechnology & Applied Microbiology
WOS Research AreaBiotechnology & Applied Microbiology
WOS KeywordHEPATOCELLULAR-CARCINOMA CELLS ; SOLID TUMORS ; MATRIX METALLOPROTEINASES ; STEM/PROGENITOR CELLS ; 3-DIMENSIONAL CULTURE ; COMPOSITE HYDROGELS ; INITIATING CELLS ; DRUG-RESISTANCE ; HYALURONIC-ACID ; SELF-RENEWAL
AbstractCancer stem cells (CSCs) are most likely the reason of cancer reoccurrence and metastasis. For further elucidation of the mechanism underlying the characteristics of CSCs, it is necessary to develop efficient culture systems to culture and expand CSCs. In this study, a three-dimensional (3D) culture system based on alginate gel (ALG) beads was reported to enrich CSCs. Two cell lines derived from different histologic origins were encapsulated in ALG beads respectively and the expansion of CSCs was investigated. Compared with two-dimensional (2D) culture, the proportion of cells with CSC-like phenotypes was significantly increased in ALG beads. Expression levels of CSC-related genes were greater in ALG beads than in 2D culture. The increase of CSC proportion after being cultured within ALG beads was further confirmed by enhanced tumorigenicity in vivo. Moreover, increased metastasis ability and higher anti-cancer drug resistance were also observed in 3D-cultured cells. Furthermore, we found that it was hypoxia, through the upregulation of hypoxia-inducible factors (HIFs) that occurred in ALG beads to induce the increasing of CSC proportion. Therefore, ALG bead was an efficient culture system for CSC enrichment, which might provide a useful platform for CSC research and promote the development of new anti-cancer therapies targeting CSCs. (C) 2014 Elsevier B.V. All rights reserved.
Language英语
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
URL查看原文
WOS IDWOS:000333811200001
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119749
Collection中国科学院大连化学物理研究所
Corresponding AuthorSun GW(孙广炜); Ma XJ(马小军)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Med Univ, Hosp 2, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Xu, Xiao-xi,Liu, Chang,Liu, Yang,et al. Enrichment of cancer stem cell-like cells by culture in alginate gel beads[J]. JOURNAL OF BIOTECHNOLOGY,2014,177:1-12.
APA Xu, Xiao-xi.,Liu, Chang.,Liu, Yang.,Yang, Li.,Li, Nan.,...&马小军.(2014).Enrichment of cancer stem cell-like cells by culture in alginate gel beads.JOURNAL OF BIOTECHNOLOGY,177,1-12.
MLA Xu, Xiao-xi,et al."Enrichment of cancer stem cell-like cells by culture in alginate gel beads".JOURNAL OF BIOTECHNOLOGY 177(2014):1-12.
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