中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: Large-Scale Quantification of Single Amino-Acid Variations by a Variation-Associated Database Search Strategy
作者: Song, Chunxia1, 2;  Wang, Fangjun1;  Cheng, Kai1;  Wei, Xiaoluan1;  Bian, Yangyang1;  Wang, Keyun1;  Tan, Yexiong3;  Wang, Hongyang3;  Ye, Mingliang1;  Zou, Hanfa1
通讯作者: 叶明亮 ;  邹汉法
关键词: single amino-acid variations ;  quantification ;  proteomics ;  liver cancer
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2014
DOI: 10.1021/pr400544j
卷: 13, 期:1, 页:241-248
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 待补充
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
资助者: 1,1
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Global quantification of the single amino-acid variations (SAAVs) is essential to investigate the roles of SAAVs in disease progression. However, few efforts have been made on this issue due to the lack of high throughput approach. Here we presented a strategy by integration of the stable isotope dimethyl labeling with variation-associated database search to globally quantify the SAAVs at the first time. A protein database containing 87 745 amino acid variant sequences and 73 910 UniProtKB/Swiss-Prot canonical protein entries was constructed for database search, and higher energy collisional dissociation combined with collision-induced dissociation fragmentation modes were applied to improve the quantification coverage of SAAVs. Compared with target proteomics in which only a few sites could be quantified, as many as 282 unique SAAVs sites were quantified between hepatocellular carcinoma (HCC) and normal human liver tissues-by our strategy. The variation rates in different samples were evaluated, and some interesting SAAVs with significant increase normalized quantification ratios, such as T1406N in CPS1 and S197R in HTATIP2, were observed to highly associate with HCC progression. Therefore, the newly developed strategy enables the large-scale comparative analysis of variations at the protein level and holds a promising future in the research related to variations.
关键词[WOS]: GENOME-WIDE ASSOCIATION ;  HEPATOCELLULAR-CARCINOMA ;  PHOSPHOPROTEOME ANALYSIS ;  PEPTIDE IDENTIFICATION ;  SHOTGUN PROTEOMICS ;  CELL-LINES ;  SUSCEPTIBILITY ;  EXPRESSION ;  MUTATIONS ;  GROWTH
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000329472700024
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119756
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2014mCoimJiJ2V.PDF(387KB)----开放获取View Download

作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.SINOPEC Res Inst Petr Proc, Beijing 100083, Peoples R China
3.Second Mil Med Univ, Int Cooperat Lab Signal Transduct, Eastern Hepatobiliary Surg Inst, Shanghai 200438, Peoples R China

Recommended Citation:
Song, Chunxia,Wang, Fangjun,Cheng, Kai,et al. Large-Scale Quantification of Single Amino-Acid Variations by a Variation-Associated Database Search Strategy[J]. JOURNAL OF PROTEOME RESEARCH,2014,13(1):241-248.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Song, Chunxia]'s Articles
 [Wang, Fangjun]'s Articles
 [Cheng, Kai]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Song, Chunxia]‘s Articles
 [Wang, Fangjun]‘s Articles
 [Cheng, Kai]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2014mCoimJiJ2V.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace