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学科主题物理化学
Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides
Huang, Guang1,2,3; Sun, Zhen2,3; Qin, Hongqiang2; Zhao, Liang2; Xiong, Zhichao2,4; Peng, Xiaojun1; Ou, Junjie2; Zou, Hanfa2; Ou JJ(欧俊杰); Zou HF(邹汉法)
刊名ANALYST
2014
ISSN0003-2654
DOI10.1039/c4an00076e
139期:9页:2199-2206
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1809
产权排名待补充
WOS标题词Science & Technology ; Physical Sciences
资助者2,1 ; 2,1 ; 2,1 ; 2,1
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]FRAGMENTATION CHAIN TRANSFER ; N-GLYCOSYLATED PROTEINS ; MASS-SPECTROMETRY ; SELECTIVE ENRICHMENT ; RADICAL POLYMERIZATION ; SILICA NANOPARTICLES ; LINKED GLYCOPEPTIDES ; BIOMARKER DISCOVERY ; MESOPOROUS SILICA ; FACILE SYNTHESIS
英文摘要Hydrazide chemistry is a powerful technique in glycopeptides enrichment. However, the low density of the monolayer hydrazine groups on the conventional hydrazine-functionalized magnetic nanoparticles limits the efficiency of glycopeptides enrichment. Herein, a novel magnetic nanoparticle grafted with poly(glycidyl methacrylate) (GMA) brushes was fabricated via reversible addition-fragmentation chain transfer (RAFT) polymerization, and a large amount of hydrazine groups were further introduced to the GMA brushes by ring-opening the epoxy groups with hydrazine hydrate. The resulting magnetic nanoparticles (denoted as Fe3O4@SiO2@GMA-NHNH2) demonstrated the high specificity of capturing glycopeptides from a tryptic digest of the sample comprising a standard non-glycosylated protein bovine serum albumin (BSA) and four standard glycoproteins with a weight ratio of 50 : 1, and the detection limit was as low as 130 fmol. In the analysis of a real complex biological sample, the tryptic digest of hepatocellular carcinoma, 179 glycosites were identified by the Fe3O4@SiO2@GMA-NHNH2 nanoparticles, surpassing that of 68 glycosites by Fe3O4@SiO2-single-NHNH2 (with monolayer hydrazine groups on the surface). It can be expected that the magnetic nanoparticles modified with hydrazine functionalized polymer brushes via RAFT technique will improve the specificity and the binding capacity of glycopeptides from complex samples, and show great potential in the analysis of protein glycosylation in biological samples.
语种英语
资助者2,1 ; 2,1 ; 2,1 ; 2,1
原文出处查看原文
WOS记录号WOS:000334176400024
引用统计
被引频次:29[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119763
专题中国科学院大连化学物理研究所
通讯作者Ou JJ(欧俊杰); Zou HF(邹汉法)
作者单位1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 20023, Peoples R China
推荐引用方式
GB/T 7714
Huang, Guang,Sun, Zhen,Qin, Hongqiang,et al. Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides[J]. ANALYST,2014,139(9):2199-2206.
APA Huang, Guang.,Sun, Zhen.,Qin, Hongqiang.,Zhao, Liang.,Xiong, Zhichao.,...&邹汉法.(2014).Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides.ANALYST,139(9),2199-2206.
MLA Huang, Guang,et al."Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides".ANALYST 139.9(2014):2199-2206.
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