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学科主题: 物理化学
题名: Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides
作者: Huang, Guang1, 2, 3;  Sun, Zhen2, 3;  Qin, Hongqiang2;  Zhao, Liang2;  Xiong, Zhichao2, 4;  Peng, Xiaojun1;  Ou, Junjie2;  Zou, Hanfa2
通讯作者: 欧俊杰 ;  邹汉法
刊名: ANALYST
发表日期: 2014
DOI: 10.1039/c4an00076e
卷: 139, 期:9, 页:2199-2206
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 待补充
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
资助者: 2,1
研究领域[WOS]: Chemistry
英文摘要: Hydrazide chemistry is a powerful technique in glycopeptides enrichment. However, the low density of the monolayer hydrazine groups on the conventional hydrazine-functionalized magnetic nanoparticles limits the efficiency of glycopeptides enrichment. Herein, a novel magnetic nanoparticle grafted with poly(glycidyl methacrylate) (GMA) brushes was fabricated via reversible addition-fragmentation chain transfer (RAFT) polymerization, and a large amount of hydrazine groups were further introduced to the GMA brushes by ring-opening the epoxy groups with hydrazine hydrate. The resulting magnetic nanoparticles (denoted as Fe3O4@SiO2@GMA-NHNH2) demonstrated the high specificity of capturing glycopeptides from a tryptic digest of the sample comprising a standard non-glycosylated protein bovine serum albumin (BSA) and four standard glycoproteins with a weight ratio of 50 : 1, and the detection limit was as low as 130 fmol. In the analysis of a real complex biological sample, the tryptic digest of hepatocellular carcinoma, 179 glycosites were identified by the Fe3O4@SiO2@GMA-NHNH2 nanoparticles, surpassing that of 68 glycosites by Fe3O4@SiO2-single-NHNH2 (with monolayer hydrazine groups on the surface). It can be expected that the magnetic nanoparticles modified with hydrazine functionalized polymer brushes via RAFT technique will improve the specificity and the binding capacity of glycopeptides from complex samples, and show great potential in the analysis of protein glycosylation in biological samples.
关键词[WOS]: FRAGMENTATION CHAIN TRANSFER ;  N-GLYCOSYLATED PROTEINS ;  MASS-SPECTROMETRY ;  SELECTIVE ENRICHMENT ;  RADICAL POLYMERIZATION ;  SILICA NANOPARTICLES ;  LINKED GLYCOPEPTIDES ;  BIOMARKER DISCOVERY ;  MESOPOROUS SILICA ;  FACILE SYNTHESIS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000334176400024
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119763
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 20023, Peoples R China

Recommended Citation:
Huang, Guang,Sun, Zhen,Qin, Hongqiang,et al. Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides[J]. ANALYST,2014,139(9):2199-2206.
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