DICP OpenIR
Subject Area物理化学
Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides
Huang, Guang1,2,3; Sun, Zhen2,3; Qin, Hongqiang2; Zhao, Liang2; Xiong, Zhichao2,4; Peng, Xiaojun1; Ou, Junjie2; Zou, Hanfa2; Ou JJ(欧俊杰); Zou HF(邹汉法)
Source PublicationANALYST
2014
ISSN0003-2654
DOI10.1039/c4an00076e
Volume139Issue:9Pages:2199-2206
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1809
Contribution Rank待补充
WOS HeadingsScience & Technology ; Physical Sciences
Funding Organization2,1 ; 2,1 ; 2,1 ; 2,1
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordFRAGMENTATION CHAIN TRANSFER ; N-GLYCOSYLATED PROTEINS ; MASS-SPECTROMETRY ; SELECTIVE ENRICHMENT ; RADICAL POLYMERIZATION ; SILICA NANOPARTICLES ; LINKED GLYCOPEPTIDES ; BIOMARKER DISCOVERY ; MESOPOROUS SILICA ; FACILE SYNTHESIS
AbstractHydrazide chemistry is a powerful technique in glycopeptides enrichment. However, the low density of the monolayer hydrazine groups on the conventional hydrazine-functionalized magnetic nanoparticles limits the efficiency of glycopeptides enrichment. Herein, a novel magnetic nanoparticle grafted with poly(glycidyl methacrylate) (GMA) brushes was fabricated via reversible addition-fragmentation chain transfer (RAFT) polymerization, and a large amount of hydrazine groups were further introduced to the GMA brushes by ring-opening the epoxy groups with hydrazine hydrate. The resulting magnetic nanoparticles (denoted as Fe3O4@SiO2@GMA-NHNH2) demonstrated the high specificity of capturing glycopeptides from a tryptic digest of the sample comprising a standard non-glycosylated protein bovine serum albumin (BSA) and four standard glycoproteins with a weight ratio of 50 : 1, and the detection limit was as low as 130 fmol. In the analysis of a real complex biological sample, the tryptic digest of hepatocellular carcinoma, 179 glycosites were identified by the Fe3O4@SiO2@GMA-NHNH2 nanoparticles, surpassing that of 68 glycosites by Fe3O4@SiO2-single-NHNH2 (with monolayer hydrazine groups on the surface). It can be expected that the magnetic nanoparticles modified with hydrazine functionalized polymer brushes via RAFT technique will improve the specificity and the binding capacity of glycopeptides from complex samples, and show great potential in the analysis of protein glycosylation in biological samples.
Language英语
Funding Organization2,1 ; 2,1 ; 2,1 ; 2,1
URL查看原文
WOS IDWOS:000334176400024
Citation statistics
Cited Times:31[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119763
Collection中国科学院大连化学物理研究所
Corresponding AuthorOu JJ(欧俊杰); Zou HF(邹汉法)
Affiliation1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 20023, Peoples R China
Recommended Citation
GB/T 7714
Huang, Guang,Sun, Zhen,Qin, Hongqiang,et al. Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides[J]. ANALYST,2014,139(9):2199-2206.
APA Huang, Guang.,Sun, Zhen.,Qin, Hongqiang.,Zhao, Liang.,Xiong, Zhichao.,...&邹汉法.(2014).Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides.ANALYST,139(9),2199-2206.
MLA Huang, Guang,et al."Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides".ANALYST 139.9(2014):2199-2206.
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