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学科主题: 物理化学
题名: Large-Scale Proteome Quantification of Hepatocellular Carcinoma Tissues by a Three-Dimensional Liquid Chromatography Strategy Integrated with Sample Preparation
作者: Xu, Bo1;  Wang, Fangjun1;  Song, Chunxia1;  Sun, Zhen1;  Cheng, Kai1;  Tan, Yexiong2;  Wang, Hongyang2;  Zou, Hanfa1
通讯作者: 王方军 ;  邹汉法
关键词: Human liver ;  hepatocellular carcinoma ;  proteome and phosphoproteome quantification ;  three-dimensional liquid chromatography separation
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2014-08-01
DOI: 10.1021/pr500200s
卷: 13, 期:8, 页:3645-3654
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 待补充
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
资助者: 1,1
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Hepatocellular carcinoma is one of the most fatal cancers worldwide. In this study, a reversed-phase strong cation exchange reversed-phase three-dimensional liquid chromatography strategy was established and coupled with mass spectrometry to investigate the differential proteome expression of HCC and normal liver tissues. In total, 2759 proteins were reliably quantified, of which, 648 proteins were dysregulated more than 3-fold in HCC liver tissues. Some important proteins that relate to HCC pathology were significantly dysregulated, such as NAT2 and AKR1B10. Furthermore, 2307 phosphorylation sites from 1264 phosphoproteins were obtained in our previous phosphoproteome quantification, and the non-phosphorylated counterparts of 445 phosphoproteins with 983 phosphorylation sites were reliably quantified in this work. It was observed that 337 (34%) phosphorylation sites exhibit significantly different expression trends from that of their corresponding of HCC were nonphosphoproteins. Some novel phosphorylation sites with important biological functions in the progression reliably quantified, such as the significant downregulation of pT185 for ERK2 and pY204 for ERK1.
关键词[WOS]: PHOSPHOPROTEOME ANALYSIS ;  QUANTITATIVE PROTEOMICS ;  EXPRESSION ;  CANCER ;  DISEASE ;  CELL ;  IDENTIFICATION ;  THROUGHPUT ;  SEPARATION ;  PATHWAYS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000339983600014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119767
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Second Mil Med Univ, Eastern Hepatobiliary Surg Inst, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China

Recommended Citation:
Xu, Bo,Wang, Fangjun,Song, Chunxia,et al. Large-Scale Proteome Quantification of Hepatocellular Carcinoma Tissues by a Three-Dimensional Liquid Chromatography Strategy Integrated with Sample Preparation[J]. JOURNAL OF PROTEOME RESEARCH,2014,13(8):3645-3654.
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