DICP OpenIR
Subject Area物理化学
Large-Scale Proteome Quantification of Hepatocellular Carcinoma Tissues by a Three-Dimensional Liquid Chromatography Strategy Integrated with Sample Preparation
Xu, Bo1; Wang, Fangjun1; Song, Chunxia1; Sun, Zhen1; Cheng, Kai1; Tan, Yexiong2; Wang, Hongyang2; Zou, Hanfa1; Wang FJ(王方军); Zou HF(邹汉法)
KeywordHuman Liver Hepatocellular Carcinoma Proteome And phosphoProteome Quantification Three-dimensional Liquid Chromatography Separation
Source PublicationJOURNAL OF PROTEOME RESEARCH
2014-08-01
ISSN1535-3893
DOI10.1021/pr500200s
Volume13Issue:8Pages:3645-3654
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1809
Contribution Rank待补充
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordPHOSPHOPROTEOME ANALYSIS ; QUANTITATIVE PROTEOMICS ; EXPRESSION ; CANCER ; DISEASE ; CELL ; IDENTIFICATION ; THROUGHPUT ; SEPARATION ; PATHWAYS
AbstractHepatocellular carcinoma is one of the most fatal cancers worldwide. In this study, a reversed-phase strong cation exchange reversed-phase three-dimensional liquid chromatography strategy was established and coupled with mass spectrometry to investigate the differential proteome expression of HCC and normal liver tissues. In total, 2759 proteins were reliably quantified, of which, 648 proteins were dysregulated more than 3-fold in HCC liver tissues. Some important proteins that relate to HCC pathology were significantly dysregulated, such as NAT2 and AKR1B10. Furthermore, 2307 phosphorylation sites from 1264 phosphoproteins were obtained in our previous phosphoproteome quantification, and the non-phosphorylated counterparts of 445 phosphoproteins with 983 phosphorylation sites were reliably quantified in this work. It was observed that 337 (34%) phosphorylation sites exhibit significantly different expression trends from that of their corresponding of HCC were nonphosphoproteins. Some novel phosphorylation sites with important biological functions in the progression reliably quantified, such as the significant downregulation of pT185 for ERK2 and pY204 for ERK1.
Language英语
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
URL查看原文
WOS IDWOS:000339983600014
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119767
Collection中国科学院大连化学物理研究所
Corresponding AuthorWang FJ(王方军); Zou HF(邹汉法)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Second Mil Med Univ, Eastern Hepatobiliary Surg Inst, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China
Recommended Citation
GB/T 7714
Xu, Bo,Wang, Fangjun,Song, Chunxia,et al. Large-Scale Proteome Quantification of Hepatocellular Carcinoma Tissues by a Three-Dimensional Liquid Chromatography Strategy Integrated with Sample Preparation[J]. JOURNAL OF PROTEOME RESEARCH,2014,13(8):3645-3654.
APA Xu, Bo.,Wang, Fangjun.,Song, Chunxia.,Sun, Zhen.,Cheng, Kai.,...&邹汉法.(2014).Large-Scale Proteome Quantification of Hepatocellular Carcinoma Tissues by a Three-Dimensional Liquid Chromatography Strategy Integrated with Sample Preparation.JOURNAL OF PROTEOME RESEARCH,13(8),3645-3654.
MLA Xu, Bo,et al."Large-Scale Proteome Quantification of Hepatocellular Carcinoma Tissues by a Three-Dimensional Liquid Chromatography Strategy Integrated with Sample Preparation".JOURNAL OF PROTEOME RESEARCH 13.8(2014):3645-3654.
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