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学科主题: 物理化学
题名: Efficient purification of low molecular weight nitrogen polar compounds from the skin of Bufo bufo gargarizans Cantor by reversed-phase high performance liquid chromatography with a polar-copolymerized C18 stationary phase
作者: Li, Xiaolong1, 2, 3;  Huang, Runqi4;  Liu, Yanfang2;  Jin, Hongli1, 2, 3;  Wan, Huihui1;  Zhao, Jianqiang2;  Zhao, Weijie1;  Liang, Xinmiao2
通讯作者: 刘艳芳 ;  赵伟杰
刊名: ANALYTICAL METHODS
发表日期: 2014
DOI: 10.1039/c4ay00716f
卷: 6, 期:14, 页:5183-5190
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 待补充
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Chemistry, Analytical ;  Food Science & Technology ;  Spectroscopy
资助者: 2,1
研究领域[WOS]: Chemistry ;  Food Science & Technology ;  Spectroscopy
英文摘要: Cinobufacini (Huachansu) injections have been widely used for the treatment of various cancers in clinical applications in China. As an aqueous extract of the skin of Bufo bufo gargarizans Cantor (toad skin), cinobufacini contains many polar compounds; however the chemical profile of these polar compounds is still unclear, which increases the risk of pharmacological safety. There are very few studies on the separation and purification of polar components from toad skin, especially in the preparative scale. In this study, a unique method based on a homemade polar-modified C18 stationary phase was developed and successfully applied to separate polar compounds from toad skin. Compared with previous purification systems based on the C18 stationary phase, the poor retention problem of polar compounds was well resolved through the use of the polar-modified C18 column and good resolutions were also achieved. Seven compounds were purified and five of them (uracil, hypoxanthine, 3-hydroxy-4H-pyrazolo[4,5-d]pyridazin-7(1H)-one, thymine and bufothionine) were identified by MS, H-1 NMR and C-13 NMR. In addition, 3-hydroxy-4H-pyrazolo[4,5-d] pyridazin-7(1H)-one was identified as a new compound and hypoxanthine was found from the skin of Bufo bufo gargarizans Cantor for the first time. These results indicate that this method shows promise for the separation of polar compounds in toad skin.
关键词[WOS]: HYDROPHILIC INTERACTION CHROMATOGRAPHY ;  IONIZATION MASS-SPECTROMETRY ;  CINOBUFACINI INJECTION ;  ACTIVE BUFADIENOLIDES ;  SEPARATION ;  TOAD ;  IDENTIFICATION ;  SECRETION ;  STEROIDS ;  EXTRACT
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000338438500033
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119794
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Univ Technol, Sch Pharmaceut Sci & Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Sichuan Univ, Inst Life Sci, Chengdu 610064, Peoples R China

Recommended Citation:
Li, Xiaolong,Huang, Runqi,Liu, Yanfang,et al. Efficient purification of low molecular weight nitrogen polar compounds from the skin of Bufo bufo gargarizans Cantor by reversed-phase high performance liquid chromatography with a polar-copolymerized C18 stationary phase[J]. ANALYTICAL METHODS,2014,6(14):5183-5190.
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