DICP OpenIR
Subject Area物理化学
Efficient purification of low molecular weight nitrogen polar compounds from the skin of Bufo bufo gargarizans Cantor by reversed-phase high performance liquid chromatography with a polar-copolymerized C18 stationary phase
Li, Xiaolong1,2,3; Huang, Runqi4; Liu, Yanfang2; Jin, Hongli1,2,3; Wan, Huihui1; Zhao, Jianqiang2; Zhao, Weijie1; Liang, Xinmiao2; Liu YF(刘艳芳); Zhao WJ(赵伟杰)
Source PublicationANALYTICAL METHODS
2014
DOI10.1039/c4ay00716f
Volume6Issue:14Pages:5183-5190
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1803
Contribution Rank待补充
WOS HeadingsScience & Technology ; Physical Sciences ; Life Sciences & Biomedicine ; Technology
Funding Organization2,1 ; 2,1 ; 2,1 ; 2,1
WOS SubjectChemistry, Analytical ; Food Science & Technology ; Spectroscopy
WOS Research AreaChemistry ; Food Science & Technology ; Spectroscopy
WOS KeywordHYDROPHILIC INTERACTION CHROMATOGRAPHY ; IONIZATION MASS-SPECTROMETRY ; CINOBUFACINI INJECTION ; ACTIVE BUFADIENOLIDES ; SEPARATION ; TOAD ; IDENTIFICATION ; SECRETION ; STEROIDS ; EXTRACT
AbstractCinobufacini (Huachansu) injections have been widely used for the treatment of various cancers in clinical applications in China. As an aqueous extract of the skin of Bufo bufo gargarizans Cantor (toad skin), cinobufacini contains many polar compounds; however the chemical profile of these polar compounds is still unclear, which increases the risk of pharmacological safety. There are very few studies on the separation and purification of polar components from toad skin, especially in the preparative scale. In this study, a unique method based on a homemade polar-modified C18 stationary phase was developed and successfully applied to separate polar compounds from toad skin. Compared with previous purification systems based on the C18 stationary phase, the poor retention problem of polar compounds was well resolved through the use of the polar-modified C18 column and good resolutions were also achieved. Seven compounds were purified and five of them (uracil, hypoxanthine, 3-hydroxy-4H-pyrazolo[4,5-d]pyridazin-7(1H)-one, thymine and bufothionine) were identified by MS, H-1 NMR and C-13 NMR. In addition, 3-hydroxy-4H-pyrazolo[4,5-d] pyridazin-7(1H)-one was identified as a new compound and hypoxanthine was found from the skin of Bufo bufo gargarizans Cantor for the first time. These results indicate that this method shows promise for the separation of polar compounds in toad skin.
Language英语
Funding Organization2,1 ; 2,1 ; 2,1 ; 2,1
URL查看原文
WOS IDWOS:000338438500033
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119794
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiu YF(刘艳芳); Zhao WJ(赵伟杰)
Affiliation1.Dalian Univ Technol, Sch Pharmaceut Sci & Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Sichuan Univ, Inst Life Sci, Chengdu 610064, Peoples R China
Recommended Citation
GB/T 7714
Li, Xiaolong,Huang, Runqi,Liu, Yanfang,et al. Efficient purification of low molecular weight nitrogen polar compounds from the skin of Bufo bufo gargarizans Cantor by reversed-phase high performance liquid chromatography with a polar-copolymerized C18 stationary phase[J]. ANALYTICAL METHODS,2014,6(14):5183-5190.
APA Li, Xiaolong.,Huang, Runqi.,Liu, Yanfang.,Jin, Hongli.,Wan, Huihui.,...&赵伟杰.(2014).Efficient purification of low molecular weight nitrogen polar compounds from the skin of Bufo bufo gargarizans Cantor by reversed-phase high performance liquid chromatography with a polar-copolymerized C18 stationary phase.ANALYTICAL METHODS,6(14),5183-5190.
MLA Li, Xiaolong,et al."Efficient purification of low molecular weight nitrogen polar compounds from the skin of Bufo bufo gargarizans Cantor by reversed-phase high performance liquid chromatography with a polar-copolymerized C18 stationary phase".ANALYTICAL METHODS 6.14(2014):5183-5190.
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