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学科主题物理化学
Hydrophilic interaction chromatography for selective separation of isomeric saponins
Guo, Xiujie; Zhang, Xiuli; Guo, Zhimou; Liu, Yanfang; Shen, Aijin; Jin, Gaowa; Liang, Xinmiao; Zhang XL(张秀莉)
关键词Hydrophilic Interaction Chromatography Methanol Saponins Isomer Separation Panax Notoginseng(Burk.) Fhchen
刊名JOURNAL OF CHROMATOGRAPHY A
2014-01-17
ISSN0021-9673
DOI10.1016/j.chroma.2013.12.006
1325页:121-128
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1803
产权排名待补充
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
资助者1,1 ; 1,1 ; 1,1 ; 1,1
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]INTERACTION LIQUID-CHROMATOGRAPHY ; BONDED STATIONARY-PHASE ; MULTIPLE LINEAR-REGRESSION ; ARTIFICIAL NEURAL-NETWORK ; FLIGHT MASS-SPECTROMETRY ; PANAX-NOTOGINSENG ; DAMMARANE-SAPONINS ; SUBAMBIENT TEMPERATURES ; PLANT SAPONINS ; GINSENOSIDES
英文摘要A method based on a Click Xlon zwitterionic stationary phase was developed to separate isomeric saponins efficiently in hydrophilic interaction chromatography (HILIC) mode using methanol as a weak eluent. The retention times of a set of isomeric saponins, ginsenoside Rc (S1), ginsenoside Rb-2 (S2), and ginsenoside Rb-3 (S3) on five kinds of HILIC columns were compared using acetonitrile/water or methanol/water as mobile phase. All results indicated that the Click Xlon column showed the highest retention times of all these isomeric saponins. Then the retention behaviors of these isomeric saponins on the Click Xlon column were investigated. The results demonstrated that the retention behaviors of saponins on Click Xlon in methanol/water mobile phase were different from that in acetonitrile/water. Methanol/water binary eluent offered enhanced selectivity to these isomeric saponins (alpha(S2/S3(MeOH)) = 1.16> alpha(S3/S2(ACN)) = 1.04), and the elution order of ginsenoside Rb-2 (S2) and ginsenoside Rb-3 (S3) was reversed compared with acetonitrile/water. Moreover, methanol/water solvent has better solubility for saponins than acetonitrile/water, resulting in improved preparative peak shape, which is greatly beneficial for saponin purification. Application to the preparative separation of saponins from leaves of P. notoginseng was also investigated, and eleven saponins, including three sets of isomeric saponins with one new saponin were isolated and identified. All these results indicated that this method was efficient for analytical and preparative separation of saponins, especially for isomeric saponins, containing xylopyranosyl, arabinofuranosyl or arabinopyranosyl units. (C) 2013 Elsevier B.V. All rights reserved.
语种英语
资助者1,1 ; 1,1 ; 1,1 ; 1,1
原文出处查看原文
WOS记录号WOS:000330256200016
引用统计
被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119795
专题中国科学院大连化学物理研究所
通讯作者Zhang XL(张秀莉)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
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GB/T 7714
Guo, Xiujie,Zhang, Xiuli,Guo, Zhimou,et al. Hydrophilic interaction chromatography for selective separation of isomeric saponins[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1325:121-128.
APA Guo, Xiujie.,Zhang, Xiuli.,Guo, Zhimou.,Liu, Yanfang.,Shen, Aijin.,...&张秀莉.(2014).Hydrophilic interaction chromatography for selective separation of isomeric saponins.JOURNAL OF CHROMATOGRAPHY A,1325,121-128.
MLA Guo, Xiujie,et al."Hydrophilic interaction chromatography for selective separation of isomeric saponins".JOURNAL OF CHROMATOGRAPHY A 1325(2014):121-128.
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