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学科主题: 物理化学
题名: Hydrophilic interaction chromatography for selective separation of isomeric saponins
作者: Guo, Xiujie1;  Zhang, Xiuli1;  Guo, Zhimou1;  Liu, Yanfang1;  Shen, Aijin1;  Jin, Gaowa1;  Liang, Xinmiao1
通讯作者: 张秀莉
关键词: Hydrophilic interaction chromatography ;  Methanol ;  Saponins ;  Isomer ;  Separation ;  Panax notoginseng(Burk.) FHChen
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2014-01-17
DOI: 10.1016/j.chroma.2013.12.006
卷: 1325, 页:121-128
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 待补充
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
资助者: 1,1
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A method based on a Click Xlon zwitterionic stationary phase was developed to separate isomeric saponins efficiently in hydrophilic interaction chromatography (HILIC) mode using methanol as a weak eluent. The retention times of a set of isomeric saponins, ginsenoside Rc (S1), ginsenoside Rb-2 (S2), and ginsenoside Rb-3 (S3) on five kinds of HILIC columns were compared using acetonitrile/water or methanol/water as mobile phase. All results indicated that the Click Xlon column showed the highest retention times of all these isomeric saponins. Then the retention behaviors of these isomeric saponins on the Click Xlon column were investigated. The results demonstrated that the retention behaviors of saponins on Click Xlon in methanol/water mobile phase were different from that in acetonitrile/water. Methanol/water binary eluent offered enhanced selectivity to these isomeric saponins (alpha(S2/S3(MeOH)) = 1.16> alpha(S3/S2(ACN)) = 1.04), and the elution order of ginsenoside Rb-2 (S2) and ginsenoside Rb-3 (S3) was reversed compared with acetonitrile/water. Moreover, methanol/water solvent has better solubility for saponins than acetonitrile/water, resulting in improved preparative peak shape, which is greatly beneficial for saponin purification. Application to the preparative separation of saponins from leaves of P. notoginseng was also investigated, and eleven saponins, including three sets of isomeric saponins with one new saponin were isolated and identified. All these results indicated that this method was efficient for analytical and preparative separation of saponins, especially for isomeric saponins, containing xylopyranosyl, arabinofuranosyl or arabinopyranosyl units. (C) 2013 Elsevier B.V. All rights reserved.
关键词[WOS]: INTERACTION LIQUID-CHROMATOGRAPHY ;  BONDED STATIONARY-PHASE ;  MULTIPLE LINEAR-REGRESSION ;  ARTIFICIAL NEURAL-NETWORK ;  FLIGHT MASS-SPECTROMETRY ;  PANAX-NOTOGINSENG ;  DAMMARANE-SAPONINS ;  SUBAMBIENT TEMPERATURES ;  PLANT SAPONINS ;  GINSENOSIDES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000330256200016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119795
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Guo, Xiujie,Zhang, Xiuli,Guo, Zhimou,et al. Hydrophilic interaction chromatography for selective separation of isomeric saponins[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1325:121-128.
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