DICP OpenIR
Subject Area物理化学
Hydrophilic interaction chromatography for selective separation of isomeric saponins
Guo, Xiujie; Zhang, Xiuli; Guo, Zhimou; Liu, Yanfang; Shen, Aijin; Jin, Gaowa; Liang, Xinmiao; Zhang XL(张秀莉)
KeywordHydrophilic Interaction Chromatography Methanol Saponins Isomer Separation Panax Notoginseng(Burk.) Fhchen
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2014-01-17
ISSN0021-9673
DOI10.1016/j.chroma.2013.12.006
Volume1325Pages:121-128
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1803
Contribution Rank待补充
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordINTERACTION LIQUID-CHROMATOGRAPHY ; BONDED STATIONARY-PHASE ; MULTIPLE LINEAR-REGRESSION ; ARTIFICIAL NEURAL-NETWORK ; FLIGHT MASS-SPECTROMETRY ; PANAX-NOTOGINSENG ; DAMMARANE-SAPONINS ; SUBAMBIENT TEMPERATURES ; PLANT SAPONINS ; GINSENOSIDES
AbstractA method based on a Click Xlon zwitterionic stationary phase was developed to separate isomeric saponins efficiently in hydrophilic interaction chromatography (HILIC) mode using methanol as a weak eluent. The retention times of a set of isomeric saponins, ginsenoside Rc (S1), ginsenoside Rb-2 (S2), and ginsenoside Rb-3 (S3) on five kinds of HILIC columns were compared using acetonitrile/water or methanol/water as mobile phase. All results indicated that the Click Xlon column showed the highest retention times of all these isomeric saponins. Then the retention behaviors of these isomeric saponins on the Click Xlon column were investigated. The results demonstrated that the retention behaviors of saponins on Click Xlon in methanol/water mobile phase were different from that in acetonitrile/water. Methanol/water binary eluent offered enhanced selectivity to these isomeric saponins (alpha(S2/S3(MeOH)) = 1.16> alpha(S3/S2(ACN)) = 1.04), and the elution order of ginsenoside Rb-2 (S2) and ginsenoside Rb-3 (S3) was reversed compared with acetonitrile/water. Moreover, methanol/water solvent has better solubility for saponins than acetonitrile/water, resulting in improved preparative peak shape, which is greatly beneficial for saponin purification. Application to the preparative separation of saponins from leaves of P. notoginseng was also investigated, and eleven saponins, including three sets of isomeric saponins with one new saponin were isolated and identified. All these results indicated that this method was efficient for analytical and preparative separation of saponins, especially for isomeric saponins, containing xylopyranosyl, arabinofuranosyl or arabinopyranosyl units. (C) 2013 Elsevier B.V. All rights reserved.
Language英语
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
URL查看原文
WOS IDWOS:000330256200016
Citation statistics
Cited Times:29[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119795
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang XL(张秀莉)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Guo, Xiujie,Zhang, Xiuli,Guo, Zhimou,et al. Hydrophilic interaction chromatography for selective separation of isomeric saponins[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1325:121-128.
APA Guo, Xiujie.,Zhang, Xiuli.,Guo, Zhimou.,Liu, Yanfang.,Shen, Aijin.,...&张秀莉.(2014).Hydrophilic interaction chromatography for selective separation of isomeric saponins.JOURNAL OF CHROMATOGRAPHY A,1325,121-128.
MLA Guo, Xiujie,et al."Hydrophilic interaction chromatography for selective separation of isomeric saponins".JOURNAL OF CHROMATOGRAPHY A 1325(2014):121-128.
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