DICP OpenIR
Subject Area物理化学
Label-free cell phenotypic profiling identifies pharmacologically active compounds in two traditional Chinese medicinal plants
Zhang, Xiuli1; Deng, Huayun2; Xiao, Yuansheng1; Xue, Xingya1; Ferrie, Ann M.2; Elizabeth Tran2; Liang, Xinmiao1; Fang, Ye2; Liang XM(梁鑫淼); Ye Fang
Source PublicationRSC ADVANCES
2014
ISSN2046-2069
DOI10.1039/c4ra03609c
Volume4Issue:50Pages:26368-26377
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1803
Contribution Rank待补充
WOS HeadingsScience & Technology ; Physical Sciences
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordDYNAMIC MASS REDISTRIBUTION ; ACID METHYL-ESTER ; DRUG DISCOVERY ; NATURAL-PRODUCTS ; PAEDERIA-SCANDENS ; NICOTINIC-ACID ; BETA(2)-ADRENERGIC RECEPTOR ; ANTINOCICEPTIVE ACTIVITY ; OPTICAL BIOSENSOR ; GPR35 AGONISTS
AbstractTraditional Chinese Medicines (TCMs) are widely used in clinical practice and natural products have been a rich resource for drug discovery. Identification of pharmacologically active chemical constituents is essential to the future of TCMs and natural product-based drug discovery. Here we report a label-free cell phenotypic profiling strategy to identify active fractions and compounds of two TCM plants including Paederia scandens (Lour.) Merri. (Jishiteng in Chinese) and Millettia pachyloba Drake (Duyuteng in Chinese), and to determine their mechanisms of action. The extracts of both plants were first fractionated to 160 fractions. Label-free cell phenotypic profiling afforded by a resonant waveguide grating biosensor in a microplate were then used to identify the active fractions acting at three different cell lines including A431, A549 and HT29. Mass spectroscopy-directed purification was then used to identify and purify active compounds. NMR and mass spectroscopy were used to determine the chemical structures of the active compounds, and pharmacological assays were used to elucidate their mechanisms of action. Using this strategy, we have discovered that both medicinal plants contain moderately high amount of niacin, an agonist for hydroxyl carboxylic acid receptor-2. Literature mining further suggests that the presence of niacin in Paederia scandens may be responsible for its antidyslipidemic effect found in clinic studies. This study demonstrates the potential of label-free cell phenotypic profiling for identifying active fractions and compounds of TCMs as well as elucidating their mechanisms of action.
Language英语
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
URL查看原文
WOS IDWOS:000338434800048
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119803
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiang XM(梁鑫淼); Ye Fang
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China
2.Corning Inc, Biochem Technol Sci & Technol Div, Corning, NY 14831 USA
Recommended Citation
GB/T 7714
Zhang, Xiuli,Deng, Huayun,Xiao, Yuansheng,et al. Label-free cell phenotypic profiling identifies pharmacologically active compounds in two traditional Chinese medicinal plants[J]. RSC ADVANCES,2014,4(50):26368-26377.
APA Zhang, Xiuli.,Deng, Huayun.,Xiao, Yuansheng.,Xue, Xingya.,Ferrie, Ann M..,...&Ye Fang.(2014).Label-free cell phenotypic profiling identifies pharmacologically active compounds in two traditional Chinese medicinal plants.RSC ADVANCES,4(50),26368-26377.
MLA Zhang, Xiuli,et al."Label-free cell phenotypic profiling identifies pharmacologically active compounds in two traditional Chinese medicinal plants".RSC ADVANCES 4.50(2014):26368-26377.
Files in This Item:
File Name/Size DocType Version Access License
2014VvuTlZxjGg.PDF(948KB) 开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Xiuli]'s Articles
[Deng, Huayun]'s Articles
[Xiao, Yuansheng]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Xiuli]'s Articles
[Deng, Huayun]'s Articles
[Xiao, Yuansheng]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Xiuli]'s Articles
[Deng, Huayun]'s Articles
[Xiao, Yuansheng]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.