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学科主题物理化学
Induction of epithelial-to-mesenchymal transition in proximal tubular epithelial cells on microfluidic devices
Zhou, Mengying1; Ma, Huipeng2,3; Lin, Hongli1; Qin, Jianhua2; Hongli Lin; Qin JH(秦建华)
关键词Biomimetic Of Proximal Tubule Epithelial-to-mesenchymal Transition Microfluidic Migration C3a
刊名BIOMATERIALS
2014-02-01
ISSN0142-9612
DOI10.1016/j.biomaterials.2013.10.070
35期:5页:1390-1401
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1807
产权排名待补充
WOS标题词Science & Technology ; Technology
资助者2,2 ; 2,2 ; 2,2 ; 2,2
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]FIBROSIS ; C3A ; COMPLEMENT ; KIDNEY ; NEPHROPATHY ; FIBROBLASTS ; EXPRESSION
英文摘要In proteinuric nephropathy, epithelial-to-mesenchymal transition (EMT) is an important mechanism that causes renal interstitial fibrosis. The precise role of EMT in the pathogenesis of fibrosis remains controversial, partly due to the absence of suitable in vitro or in vivo models. We developed two microfluidic and compartmental chips that reproduced the fluidic and three-dimensional microenvironment of proximal tubular epithelial cells in vivo. Using one microfluidic device, we stimulated epithelial cells with a flow of healthy human serum, heat-inactivated serum and complement C3a, which mimicked the flow of urine within the proximal tubule. We observed that epithelial cells exposed to serum proteins became apoptotic or developed a mesenchymal phenotype. Incubating cells with C3a induced similar features. However, cells exposed to heat-inactivated serum did not adopt the mesenchymal phenotype. Furthermore, we successfully recorded the cellular morphological changes and the process of transmigration into basement membrane extract during EMT in real-time using another three-dimensional microdevice. In conclusion, we have established a cell-culture system that mimics the native microenvironment of the proximal tubule to a certain extent. Our data indicates that EMT did occur in epithelial cells that were exposed to serum proteins, and C3a plays an essential role in this pathological process. (C) 2013 Elsevier Ltd. All rights reserved.
语种英语
资助者2,2 ; 2,2 ; 2,2 ; 2,2
原文出处查看原文
WOS记录号WOS:000330156100005
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119808
专题中国科学院大连化学物理研究所
通讯作者Hongli Lin; Qin JH(秦建华)
作者单位1.Dalian Med Univ, Affiliated Hosp 1, Dept Nephrol, Dalian 116011, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
3.Dalian Med Univ, Coll Med Lab, Dalian 116044, Peoples R China
推荐引用方式
GB/T 7714
Zhou, Mengying,Ma, Huipeng,Lin, Hongli,et al. Induction of epithelial-to-mesenchymal transition in proximal tubular epithelial cells on microfluidic devices[J]. BIOMATERIALS,2014,35(5):1390-1401.
APA Zhou, Mengying,Ma, Huipeng,Lin, Hongli,Qin, Jianhua,Hongli Lin,&秦建华.(2014).Induction of epithelial-to-mesenchymal transition in proximal tubular epithelial cells on microfluidic devices.BIOMATERIALS,35(5),1390-1401.
MLA Zhou, Mengying,et al."Induction of epithelial-to-mesenchymal transition in proximal tubular epithelial cells on microfluidic devices".BIOMATERIALS 35.5(2014):1390-1401.
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