DICP OpenIR
Subject Area物理化学
Induction of epithelial-to-mesenchymal transition in proximal tubular epithelial cells on microfluidic devices
Zhou, Mengying1; Ma, Huipeng2,3; Lin, Hongli1; Qin, Jianhua2; Hongli Lin; Qin JH(秦建华)
KeywordBiomimetic Of Proximal Tubule Epithelial-to-mesenchymal Transition Microfluidic Migration C3a
Source PublicationBIOMATERIALS
2014-02-01
ISSN0142-9612
DOI10.1016/j.biomaterials.2013.10.070
Volume35Issue:5Pages:1390-1401
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1807
Contribution Rank待补充
WOS HeadingsScience & Technology ; Technology
Funding Organization2,2 ; 2,2 ; 2,2 ; 2,2
WOS SubjectEngineering, Biomedical ; Materials Science, Biomaterials
WOS Research AreaEngineering ; Materials Science
WOS KeywordFIBROSIS ; C3A ; COMPLEMENT ; KIDNEY ; NEPHROPATHY ; FIBROBLASTS ; EXPRESSION
AbstractIn proteinuric nephropathy, epithelial-to-mesenchymal transition (EMT) is an important mechanism that causes renal interstitial fibrosis. The precise role of EMT in the pathogenesis of fibrosis remains controversial, partly due to the absence of suitable in vitro or in vivo models. We developed two microfluidic and compartmental chips that reproduced the fluidic and three-dimensional microenvironment of proximal tubular epithelial cells in vivo. Using one microfluidic device, we stimulated epithelial cells with a flow of healthy human serum, heat-inactivated serum and complement C3a, which mimicked the flow of urine within the proximal tubule. We observed that epithelial cells exposed to serum proteins became apoptotic or developed a mesenchymal phenotype. Incubating cells with C3a induced similar features. However, cells exposed to heat-inactivated serum did not adopt the mesenchymal phenotype. Furthermore, we successfully recorded the cellular morphological changes and the process of transmigration into basement membrane extract during EMT in real-time using another three-dimensional microdevice. In conclusion, we have established a cell-culture system that mimics the native microenvironment of the proximal tubule to a certain extent. Our data indicates that EMT did occur in epithelial cells that were exposed to serum proteins, and C3a plays an essential role in this pathological process. (C) 2013 Elsevier Ltd. All rights reserved.
Language英语
Funding Organization2,2 ; 2,2 ; 2,2 ; 2,2
URL查看原文
WOS IDWOS:000330156100005
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119808
Collection中国科学院大连化学物理研究所
Corresponding AuthorHongli Lin; Qin JH(秦建华)
Affiliation1.Dalian Med Univ, Affiliated Hosp 1, Dept Nephrol, Dalian 116011, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
3.Dalian Med Univ, Coll Med Lab, Dalian 116044, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Mengying,Ma, Huipeng,Lin, Hongli,et al. Induction of epithelial-to-mesenchymal transition in proximal tubular epithelial cells on microfluidic devices[J]. BIOMATERIALS,2014,35(5):1390-1401.
APA Zhou, Mengying,Ma, Huipeng,Lin, Hongli,Qin, Jianhua,Hongli Lin,&秦建华.(2014).Induction of epithelial-to-mesenchymal transition in proximal tubular epithelial cells on microfluidic devices.BIOMATERIALS,35(5),1390-1401.
MLA Zhou, Mengying,et al."Induction of epithelial-to-mesenchymal transition in proximal tubular epithelial cells on microfluidic devices".BIOMATERIALS 35.5(2014):1390-1401.
Files in This Item:
File Name/Size DocType Version Access License
2014wuqsZprnDr.PDF(4895KB) 开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhou, Mengying]'s Articles
[Ma, Huipeng]'s Articles
[Lin, Hongli]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhou, Mengying]'s Articles
[Ma, Huipeng]'s Articles
[Lin, Hongli]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhou, Mengying]'s Articles
[Ma, Huipeng]'s Articles
[Lin, Hongli]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.