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学科主题: 物理化学
题名: Induction of epithelial-to-mesenchymal transition in proximal tubular epithelial cells on microfluidic devices
作者: Zhou, Mengying1;  Ma, Huipeng2, 3;  Lin, Hongli1;  Qin, Jianhua2
通讯作者: Hongli Lin ;  秦建华
关键词: Biomimetic of proximal tubule ;  Epithelial-to-mesenchymal transition ;  Microfluidic ;  Migration ;  C3a
刊名: BIOMATERIALS
发表日期: 2014-02-01
DOI: 10.1016/j.biomaterials.2013.10.070
卷: 35, 期:5, 页:1390-1401
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 待补充
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Engineering, Biomedical ;  Materials Science, Biomaterials
资助者: 2,2
研究领域[WOS]: Engineering ;  Materials Science
英文摘要: In proteinuric nephropathy, epithelial-to-mesenchymal transition (EMT) is an important mechanism that causes renal interstitial fibrosis. The precise role of EMT in the pathogenesis of fibrosis remains controversial, partly due to the absence of suitable in vitro or in vivo models. We developed two microfluidic and compartmental chips that reproduced the fluidic and three-dimensional microenvironment of proximal tubular epithelial cells in vivo. Using one microfluidic device, we stimulated epithelial cells with a flow of healthy human serum, heat-inactivated serum and complement C3a, which mimicked the flow of urine within the proximal tubule. We observed that epithelial cells exposed to serum proteins became apoptotic or developed a mesenchymal phenotype. Incubating cells with C3a induced similar features. However, cells exposed to heat-inactivated serum did not adopt the mesenchymal phenotype. Furthermore, we successfully recorded the cellular morphological changes and the process of transmigration into basement membrane extract during EMT in real-time using another three-dimensional microdevice. In conclusion, we have established a cell-culture system that mimics the native microenvironment of the proximal tubule to a certain extent. Our data indicates that EMT did occur in epithelial cells that were exposed to serum proteins, and C3a plays an essential role in this pathological process. (C) 2013 Elsevier Ltd. All rights reserved.
关键词[WOS]: FIBROSIS ;  C3A ;  COMPLEMENT ;  KIDNEY ;  NEPHROPATHY ;  FIBROBLASTS ;  EXPRESSION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000330156100005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119808
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Med Univ, Affiliated Hosp 1, Dept Nephrol, Dalian 116011, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
3.Dalian Med Univ, Coll Med Lab, Dalian 116044, Peoples R China

Recommended Citation:
Zhou, Mengying,Ma, Huipeng,Lin, Hongli,et al. Induction of epithelial-to-mesenchymal transition in proximal tubular epithelial cells on microfluidic devices[J]. BIOMATERIALS,2014,35(5):1390-1401.
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