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学科主题: 物理化学
题名: Purification of bufadienolides from the skin of Bufo bufo gargarizans Cantor with positively charged C18 column
作者: Li, Xiaolong1, 2, 3;  Guo, Zhimou1;  Wang, Chaoran1;  Shen, Aijin1;  Liu, Yanfang1;  Zhang, Xiuli1;  Zhao, Weijie2;  Liang, Xinmiao1
通讯作者: 刘艳芳 ;  梁鑫淼
关键词: Purification ;  Bufadienolides ;  Bufo bufo gargarizans Cantor ;  Reversed-phase liquid chromatography ;  Positively charged C18
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2014-04-15
DOI: 10.1016/j.jpba.2014.01.002
卷: 92, 页:105-113
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 待补充
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine
类目[WOS]: Chemistry, Analytical ;  Pharmacology & Pharmacy
资助者: 1,1
研究领域[WOS]: Chemistry ;  Pharmacology & Pharmacy
英文摘要: As a kind of promising anticancer compounds, the preparation of bufadienolides is a hot study spot. However, due to the complexity of biological sample, the purification of bufadienolides from a crude sample (toad skin) is a tough work. In this paper, we reported a new way based on positively charged C18 material (XCharge C18) to quickly separate and purify bufadienolides from toad skin. By this method, the different ionic feature of the amino acid conjugated bufadienolides (AACBs) and the free form bufadienolides (AAUBs) was firstly utilized to obtain distinct separation selectivity on the XCharge C18 column. Additionally, the peak tailing problem of AACBs on conventional C18 was resolved and better resolutions were achieved on the XCharge C18, thus, two kinds of bufadienolides on one column were successfully purified respectively. Taking F13 as an example, the method was validated by liquid chromatography-mass spectrometry (LC-MS), and then 4 AACBs as well as 4 AAUBs were simultaneously purified by preparative XCharge C18. In addition, the application of this method in other fractions was also validated. The results suggested that the developed method is a practical and promising tool for efficient separation and purification of bufadienolides from toad skin. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: FLIGHT MASS-SPECTROMETRY ;  DRUG CHAN-SU ;  LIQUID-CHROMATOGRAPHY ;  CINOBUFACINI INJECTION ;  TOAD SKIN ;  STEROIDS ;  SEPARATION ;  MEDICINE ;  BIOTRANSFORMATION ;  CINOBUFOTALIN
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000335487300015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119810
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Sch Pharmaceut Sci & Technol, Dalian 116024, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Li, Xiaolong,Guo, Zhimou,Wang, Chaoran,et al. Purification of bufadienolides from the skin of Bufo bufo gargarizans Cantor with positively charged C18 column[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2014,92:105-113.
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