DICP OpenIR
Subject Area物理化学
Purification of bufadienolides from the skin of Bufo bufo gargarizans Cantor with positively charged C18 column
Li, Xiaolong1,2,3; Guo, Zhimou1; Wang, Chaoran1; Shen, Aijin1; Liu, Yanfang1; Zhang, Xiuli1; Zhao, Weijie2; Liang, Xinmiao1; Liu YF(刘艳芳); Liang XM(梁鑫淼)
KeywordPurification Bufadienolides Bufo Bufo Gargarizans Cantor Reversed-phase Liquid Chromatography Positively Charged C18
Source PublicationJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2014-04-15
DOI10.1016/j.jpba.2014.01.002
Volume92Pages:105-113
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1803
Contribution Rank待补充
WOS HeadingsScience & Technology ; Physical Sciences ; Life Sciences & Biomedicine
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
WOS SubjectChemistry, Analytical ; Pharmacology & Pharmacy
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS KeywordFLIGHT MASS-SPECTROMETRY ; DRUG CHAN-SU ; LIQUID-CHROMATOGRAPHY ; CINOBUFACINI INJECTION ; TOAD SKIN ; STEROIDS ; SEPARATION ; MEDICINE ; BIOTRANSFORMATION ; CINOBUFOTALIN
AbstractAs a kind of promising anticancer compounds, the preparation of bufadienolides is a hot study spot. However, due to the complexity of biological sample, the purification of bufadienolides from a crude sample (toad skin) is a tough work. In this paper, we reported a new way based on positively charged C18 material (XCharge C18) to quickly separate and purify bufadienolides from toad skin. By this method, the different ionic feature of the amino acid conjugated bufadienolides (AACBs) and the free form bufadienolides (AAUBs) was firstly utilized to obtain distinct separation selectivity on the XCharge C18 column. Additionally, the peak tailing problem of AACBs on conventional C18 was resolved and better resolutions were achieved on the XCharge C18, thus, two kinds of bufadienolides on one column were successfully purified respectively. Taking F13 as an example, the method was validated by liquid chromatography-mass spectrometry (LC-MS), and then 4 AACBs as well as 4 AAUBs were simultaneously purified by preparative XCharge C18. In addition, the application of this method in other fractions was also validated. The results suggested that the developed method is a practical and promising tool for efficient separation and purification of bufadienolides from toad skin. (C) 2014 Elsevier B.V. All rights reserved.
Language英语
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
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WOS IDWOS:000335487300015
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119810
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiu YF(刘艳芳); Liang XM(梁鑫淼)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Sch Pharmaceut Sci & Technol, Dalian 116024, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Li, Xiaolong,Guo, Zhimou,Wang, Chaoran,et al. Purification of bufadienolides from the skin of Bufo bufo gargarizans Cantor with positively charged C18 column[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2014,92:105-113.
APA Li, Xiaolong.,Guo, Zhimou.,Wang, Chaoran.,Shen, Aijin.,Liu, Yanfang.,...&梁鑫淼.(2014).Purification of bufadienolides from the skin of Bufo bufo gargarizans Cantor with positively charged C18 column.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,92,105-113.
MLA Li, Xiaolong,et al."Purification of bufadienolides from the skin of Bufo bufo gargarizans Cantor with positively charged C18 column".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 92(2014):105-113.
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