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学科主题: 物理化学
题名: A dextran-bonded stationary phase for saccharide separation
作者: Sheng, Qianying1;  Su, Xindai3;  Li, Xiuling2;  Ke, Yanxiong1;  Liang, Xinmiao1, 2
通讯作者: 柯燕雄 ;  梁鑫淼
关键词: Dextran ;  Oligosaccharide ;  Glycopeptides ;  HILIC ;  MS
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2014-06-06
DOI: 10.1016/j.chroma.2014.03.076
卷: 1345, 页:57-67
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 待补充
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
资助者: 2,3
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A-saccharide-based stationary phase for hydrophilic interaction liquid chromatography (HILIC) is presented. The method uses carbonyl di-imidazole (CDI) as a cross-linker for dextran in aqueous solution. Different from the traditional immobilized saccharide stationary phase, this method was using a simple way to bond the high molecular of polysaccharide immobilized on the silica gel. The new method avoided the time-consuming process but had a very considerable loading result. With silica-based surface bonded, it will be possible to develop well-defined surface modifications that promote the hydrophilic with dextran and high mechanical strength with silica. For all tested compounds, including polar compounds and carbohydrates, this dextran-bonded stationary phase performed well in terms of separation efficiency and column stability, and the retention mechanism matched that of typical HILIC retention. Moreover, good selectivity was achieved in the separation of oligosaccharides and glycopeptides. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: HYDROPHILIC-INTERACTION CHROMATOGRAPHY ;  INTERACTION LIQUID-CHROMATOGRAPHY ;  LIGHT-SCATTERING DETECTION ;  MASS-SPECTROMETRY ;  REVERSED-PHASE ;  PANAX-NOTOGINSENG ;  PEPTIDE SYNTHESIS ;  POLAR COMPOUNDS ;  SILICA ;  GLYCOPEPTIDES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000336709300008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119817
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.E China Univ Sci & Technol, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, Dalian 116023, Peoples R China

Recommended Citation:
Sheng, Qianying,Su, Xindai,Li, Xiuling,et al. A dextran-bonded stationary phase for saccharide separation[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1345:57-67.
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