DICP OpenIR
Subject Area物理化学
A dextran-bonded stationary phase for saccharide separation
Sheng, Qianying1; Su, Xindai3; Li, Xiuling2; Ke, Yanxiong1; Liang, Xinmiao1,2; Ke YX(柯燕雄); Liang XM(梁鑫淼)
KeywordDextran Oligosaccharide Glycopeptides Hilic Ms
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2014-06-06
ISSN0021-9673
DOI10.1016/j.chroma.2014.03.076
Volume1345Pages:57-67
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1803
Contribution Rank待补充
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Funding Organization2,3 ; 2,3 ; 2,3 ; 2,3
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordHYDROPHILIC-INTERACTION CHROMATOGRAPHY ; INTERACTION LIQUID-CHROMATOGRAPHY ; LIGHT-SCATTERING DETECTION ; MASS-SPECTROMETRY ; REVERSED-PHASE ; PANAX-NOTOGINSENG ; PEPTIDE SYNTHESIS ; POLAR COMPOUNDS ; SILICA ; GLYCOPEPTIDES
AbstractA-saccharide-based stationary phase for hydrophilic interaction liquid chromatography (HILIC) is presented. The method uses carbonyl di-imidazole (CDI) as a cross-linker for dextran in aqueous solution. Different from the traditional immobilized saccharide stationary phase, this method was using a simple way to bond the high molecular of polysaccharide immobilized on the silica gel. The new method avoided the time-consuming process but had a very considerable loading result. With silica-based surface bonded, it will be possible to develop well-defined surface modifications that promote the hydrophilic with dextran and high mechanical strength with silica. For all tested compounds, including polar compounds and carbohydrates, this dextran-bonded stationary phase performed well in terms of separation efficiency and column stability, and the retention mechanism matched that of typical HILIC retention. Moreover, good selectivity was achieved in the separation of oligosaccharides and glycopeptides. (C) 2014 Elsevier B.V. All rights reserved.
Language英语
Funding Organization2,3 ; 2,3 ; 2,3 ; 2,3
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WOS IDWOS:000336709300008
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119817
Collection中国科学院大连化学物理研究所
Corresponding AuthorKe YX(柯燕雄); Liang XM(梁鑫淼)
Affiliation1.E China Univ Sci & Technol, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Sheng, Qianying,Su, Xindai,Li, Xiuling,et al. A dextran-bonded stationary phase for saccharide separation[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1345:57-67.
APA Sheng, Qianying.,Su, Xindai.,Li, Xiuling.,Ke, Yanxiong.,Liang, Xinmiao.,...&梁鑫淼.(2014).A dextran-bonded stationary phase for saccharide separation.JOURNAL OF CHROMATOGRAPHY A,1345,57-67.
MLA Sheng, Qianying,et al."A dextran-bonded stationary phase for saccharide separation".JOURNAL OF CHROMATOGRAPHY A 1345(2014):57-67.
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