中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: Ti4+-immobilized multilayer polysaccharide coated magnetic nanoparticles for highly selective enrichment of phosphopeptides
作者: Xiong, Zhichao1, 2;  Zhang, Lingyi1;  Fang, Chunli2;  Zhang, Quanqing2;  Ji, Yongsheng2, 3;  Zhang, Zhang2;  Zhang, Weibing1, 2;  Zou, Hanfa2
通讯作者: 张维冰 ;  邹汉法
刊名: JOURNAL OF MATERIALS CHEMISTRY B
发表日期: 2014
DOI: 10.1039/c4tb00479e
卷: 2, 期:28, 页:4473-4480
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 待补充
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Materials Science, Biomaterials
资助者: 2,1
研究领域[WOS]: Materials Science
英文摘要: Highly selective and efficient enrichment of trace phosphorylated proteins or peptides from complex biological samples is of profound significance for the discovery of disease biomarkers in biological systems. In this study, a novel immobilized metal affinity chromatography (IMAC) material has been synthesized to improve the enrichment specificity and sensitivity for phosphopeptides by introducing a titanium phosphate moiety on a multilayer polysaccharide (hyaluronate (HA) and chitosan (CS)) coated Fe3O4@SiO2 nanoparticle (denoted as Fe3O4@ SiO2@(HA/CS)(10)-Ti4+ IMAC). The thicker multilayer polysaccharide endows excellent hydrophilic properties and a higher binding capacity of the titanium ion to the IMAC material. Due to the combination of uniform magnetic properties, highly hydrophilic properties and enhanced binding capacity of the titanium ion, the Fe3O4@SiO2@(HA/CS)(10)-Ti4+ nanoparticle possesses many merits, such as high selectivity for phosphopeptides (phosphopeptides/non-phosphopeptides at a molar ratio of 1 : 2000), extreme detection sensitivity (0.5 fmol), large binding capacity (100 mg g(-1)) high enrichment recovery (85.45%) and rapid magnetic separation (within 10 s). Moreover, the as- prepared IMAC nanoparticle provides effective enrichment of phosphopeptides from real samples (human serum and nonfat milk), showing great potential as a tool for the detection and identification of low-abundance phosphopeptides in biological samples.
关键词[WOS]: ION AFFINITY-CHROMATOGRAPHY ;  IMMOBILIZED METAL-IONS ;  MALDI-TOF MS ;  PHOSPHOPROTEOME ANALYSIS ;  NANOCRYSTAL CLUSTERS ;  SAMPLE PREPARATION ;  MASS-SPECTROMETRY ;  IN-VIVO ;  MICROSPHERES ;  PROTEINS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000338646000010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119821
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2014YwvhW03NYL.PDF(849KB)----开放获取View Download

作者单位: 1.E China Univ Sci & Technol, Dept Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 1160237, Peoples R China
3.Henan Univ Tradit Chinese Med, Coll Pharm, Zhengzhou 450000, Peoples R China

Recommended Citation:
Xiong, Zhichao,Zhang, Lingyi,Fang, Chunli,et al. Ti4+-immobilized multilayer polysaccharide coated magnetic nanoparticles for highly selective enrichment of phosphopeptides[J]. JOURNAL OF MATERIALS CHEMISTRY B,2014,2(28):4473-4480.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Xiong, Zhichao]'s Articles
 [Zhang, Lingyi]'s Articles
 [Fang, Chunli]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Xiong, Zhichao]‘s Articles
 [Zhang, Lingyi]‘s Articles
 [Fang, Chunli]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2014YwvhW03NYL.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace