DICP OpenIR
Subject Area物理化学
Ti4+-immobilized multilayer polysaccharide coated magnetic nanoparticles for highly selective enrichment of phosphopeptides
Xiong, Zhichao1,2; Zhang, Lingyi1; Fang, Chunli2; Zhang, Quanqing2; Ji, Yongsheng2,3; Zhang, Zhang2; Zhang, Weibing1,2; Zou, Hanfa2; Zhang WB(张维冰); Zou HF(邹汉法)
Source PublicationJOURNAL OF MATERIALS CHEMISTRY B
2014
ISSN0959-9428
DOI10.1039/c4tb00479e
Volume2Issue:28Pages:4473-4480
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1809
Contribution Rank待补充
WOS HeadingsScience & Technology ; Technology
Funding Organization2,1 ; 2,1 ; 2,1 ; 2,1
WOS SubjectMaterials Science, Biomaterials
WOS Research AreaMaterials Science
WOS KeywordION AFFINITY-CHROMATOGRAPHY ; IMMOBILIZED METAL-IONS ; MALDI-TOF MS ; PHOSPHOPROTEOME ANALYSIS ; NANOCRYSTAL CLUSTERS ; SAMPLE PREPARATION ; MASS-SPECTROMETRY ; IN-VIVO ; MICROSPHERES ; PROTEINS
AbstractHighly selective and efficient enrichment of trace phosphorylated proteins or peptides from complex biological samples is of profound significance for the discovery of disease biomarkers in biological systems. In this study, a novel immobilized metal affinity chromatography (IMAC) material has been synthesized to improve the enrichment specificity and sensitivity for phosphopeptides by introducing a titanium phosphate moiety on a multilayer polysaccharide (hyaluronate (HA) and chitosan (CS)) coated Fe3O4@SiO2 nanoparticle (denoted as Fe3O4@ SiO2@(HA/CS)(10)-Ti4+ IMAC). The thicker multilayer polysaccharide endows excellent hydrophilic properties and a higher binding capacity of the titanium ion to the IMAC material. Due to the combination of uniform magnetic properties, highly hydrophilic properties and enhanced binding capacity of the titanium ion, the Fe3O4@SiO2@(HA/CS)(10)-Ti4+ nanoparticle possesses many merits, such as high selectivity for phosphopeptides (phosphopeptides/non-phosphopeptides at a molar ratio of 1 : 2000), extreme detection sensitivity (0.5 fmol), large binding capacity (100 mg g(-1)) high enrichment recovery (85.45%) and rapid magnetic separation (within 10 s). Moreover, the as- prepared IMAC nanoparticle provides effective enrichment of phosphopeptides from real samples (human serum and nonfat milk), showing great potential as a tool for the detection and identification of low-abundance phosphopeptides in biological samples.
Language英语
Funding Organization2,1 ; 2,1 ; 2,1 ; 2,1
URL查看原文
WOS IDWOS:000338646000010
Citation statistics
Cited Times:50[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119821
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang WB(张维冰); Zou HF(邹汉法)
Affiliation1.E China Univ Sci & Technol, Dept Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 1160237, Peoples R China
3.Henan Univ Tradit Chinese Med, Coll Pharm, Zhengzhou 450000, Peoples R China
Recommended Citation
GB/T 7714
Xiong, Zhichao,Zhang, Lingyi,Fang, Chunli,et al. Ti4+-immobilized multilayer polysaccharide coated magnetic nanoparticles for highly selective enrichment of phosphopeptides[J]. JOURNAL OF MATERIALS CHEMISTRY B,2014,2(28):4473-4480.
APA Xiong, Zhichao.,Zhang, Lingyi.,Fang, Chunli.,Zhang, Quanqing.,Ji, Yongsheng.,...&邹汉法.(2014).Ti4+-immobilized multilayer polysaccharide coated magnetic nanoparticles for highly selective enrichment of phosphopeptides.JOURNAL OF MATERIALS CHEMISTRY B,2(28),4473-4480.
MLA Xiong, Zhichao,et al."Ti4+-immobilized multilayer polysaccharide coated magnetic nanoparticles for highly selective enrichment of phosphopeptides".JOURNAL OF MATERIALS CHEMISTRY B 2.28(2014):4473-4480.
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