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题名: Reversed Phase Monolithic Column Based Enzyme Reactor for Protein Analysis
作者: Liu Jing1, 2;  Wang Fang-Jun1;  Zhang Zhen-Bin1, 2;  Zou Han-Fa1
关键词: Reversed phase polymer-based monolithic column ;  Enzyme reactor ;  Liquid chromatography coupled with mass spectrometry
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2013
DOI: 10.3724/SP.J.1096.2013.20720
卷: 41, 期:1, 页:10-14
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: We developed a reversed phase monolithic column based enzyme reactor for protein analysis. Using the number of identified unique peptides, sequence coverage and the level of missed cleavage as the evaluation indicators, we systematically investigated the influence of buffer solution, porous structure of the column, digestion time and hydrophobicity of solid phase on the digestion performance of enzyme reactor. It had demonstrated that the reversed phase monolithic column based enzyme reactor had high digestion efficiency and might be further extended to the proteome analysis of complex samples.
关键词[WOS]: DIGESTION ;  IDENTIFICATION ;  PROTEOLYSIS ;  SYSTEM
语种: 英语
WOS记录号: WOS:000315021700002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137471
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Liu Jing,Wang Fang-Jun,Zhang Zhen-Bin,et al. Reversed Phase Monolithic Column Based Enzyme Reactor for Protein Analysis[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2013,41(1):10-14.
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