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Reversed Phase Monolithic Column Based Enzyme Reactor for Protein Analysis
Liu Jing1,2; Wang Fang-Jun1; Zhang Zhen-Bin1,2; Zou Han-Fa1
关键词Reversed Phase Polymer-based Monolithic Column Enzyme Reactor Liquid Chromatography Coupled With Mass Spectrometry
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2013
DOI10.3724/SP.J.1096.2013.20720
41期:1页:10-14
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]DIGESTION ; IDENTIFICATION ; PROTEOLYSIS ; SYSTEM
英文摘要We developed a reversed phase monolithic column based enzyme reactor for protein analysis. Using the number of identified unique peptides, sequence coverage and the level of missed cleavage as the evaluation indicators, we systematically investigated the influence of buffer solution, porous structure of the column, digestion time and hydrophobicity of solid phase on the digestion performance of enzyme reactor. It had demonstrated that the reversed phase monolithic column based enzyme reactor had high digestion efficiency and might be further extended to the proteome analysis of complex samples.
语种英语
WOS记录号WOS:000315021700002
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/137471
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
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GB/T 7714
Liu Jing,Wang Fang-Jun,Zhang Zhen-Bin,et al. Reversed Phase Monolithic Column Based Enzyme Reactor for Protein Analysis[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2013,41(1):10-14.
APA Liu Jing,Wang Fang-Jun,Zhang Zhen-Bin,&Zou Han-Fa.(2013).Reversed Phase Monolithic Column Based Enzyme Reactor for Protein Analysis.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,41(1),10-14.
MLA Liu Jing,et al."Reversed Phase Monolithic Column Based Enzyme Reactor for Protein Analysis".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 41.1(2013):10-14.
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