中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Phosphoproteome analysis of an early onset mouse model (TgCRND8) of Alzheimer's disease reveals temporal changes in neuronal and glia signaling pathways
作者: Wang, Fangjun1, 2;  Blanchard, Alexandre P.2, 3;  Elisma, Fred2;  Granger, Matthew2, 3;  Xu, Hongbin2, 3;  Bennett, Steffany A. L.2, 3;  Figeys, Daniel2;  Zou, Hanfa1
关键词: Alzheimer's disease ;  Animal model ;  Animal proteomics ;  MS ;  Phosphoproteomics ;  TgCRND8
刊名: PROTEOMICS
发表日期: 2013-04-01
DOI: 10.1002/pmic.201200415
卷: 13, 期:8, 页:1292-1305
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Sustained exposure to soluble amyloid (A42) oligomers is predicted to impair synaptic function in the hippocampal-entorhinal circuit, signaling synaptic loss and precipitating cognitive impairment in Alzheimer's disease. Regional changes in overall patterns of protein phosphorylation are likely crucial to promote transition from a presymptomatic to a symptomatic state in response to accumulating A42. Here, we used unbiased proteomic approaches to compare the phosphoproteome of presymptomatic and symptomatic TgCRND8 mice and identify network disruptions in signaling pathways implicated in the manifestation of behavioral indices of learning and memory impairment. Phosphopeptide enrichment with triple isotopic dimethylation labeling combined with online multidimensional separation and MS was used to profile phosphoproteome changes in 2- and 6-month-old TgCRND8 mice and congenic littermate controls. We identified 1026 phosphopeptides representing 1168 phosphorylation sites from 476 unique proteins. Of these, 595 phosphopeptides from 293 unique proteins were reliably quantified and 139 phosphopeptides were found to change significantly in the hippocampus of TgCRND8 mice following conversion from a presymptomatic to a symptomatic state.
关键词[WOS]: RESPONSE MEDIATOR PROTEINS ;  AMYLOID-BETA-PROTEIN ;  MYELIN BASIC-PROTEIN ;  IN-VIVO ;  QUANTITATIVE PROTEOMICS ;  TAU ;  PHOSPHORYLATION ;  AGGREGATION ;  BINDING ;  MICE
语种: 英语
WOS记录号: WOS:000317684800007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137475
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian, Peoples R China
2.Univ Ottawa, Ottawa Inst Syst Biol, Ottawa, ON K1H 8M5, Canada
3.Univ Ottawa, Neural Regenerat Lab, Dept Biochem Microbiol & Immunol, Ottawa, ON K1H 8M5, Canada

Recommended Citation:
Wang, Fangjun,Blanchard, Alexandre P.,Elisma, Fred,et al. Phosphoproteome analysis of an early onset mouse model (TgCRND8) of Alzheimer's disease reveals temporal changes in neuronal and glia signaling pathways[J]. PROTEOMICS,2013,13(8):1292-1305.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Wang, Fangjun]'s Articles
 [Blanchard, Alexandre P.]'s Articles
 [Elisma, Fred]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Wang, Fangjun]‘s Articles
 [Blanchard, Alexandre P.]‘s Articles
 [Elisma, Fred]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace