DICP OpenIR
Phosphoproteome analysis of an early onset mouse model (TgCRND8) of Alzheimer's disease reveals temporal changes in neuronal and glia signaling pathways
Wang, Fangjun1,2; Blanchard, Alexandre P.2,3; Elisma, Fred2; Granger, Matthew2,3; Xu, Hongbin2,3; Bennett, Steffany A. L.2,3; Figeys, Daniel2; Zou, Hanfa1
关键词Alzheimer's Disease Animal Model Animal Proteomics Ms Phosphoproteomics Tgcrnd8
刊名PROTEOMICS
2013-04-01
DOI10.1002/pmic.201200415
13期:8页:1292-1305
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]RESPONSE MEDIATOR PROTEINS ; AMYLOID-BETA-PROTEIN ; MYELIN BASIC-PROTEIN ; IN-VIVO ; QUANTITATIVE PROTEOMICS ; TAU ; PHOSPHORYLATION ; AGGREGATION ; BINDING ; MICE
英文摘要Sustained exposure to soluble amyloid (A42) oligomers is predicted to impair synaptic function in the hippocampal-entorhinal circuit, signaling synaptic loss and precipitating cognitive impairment in Alzheimer's disease. Regional changes in overall patterns of protein phosphorylation are likely crucial to promote transition from a presymptomatic to a symptomatic state in response to accumulating A42. Here, we used unbiased proteomic approaches to compare the phosphoproteome of presymptomatic and symptomatic TgCRND8 mice and identify network disruptions in signaling pathways implicated in the manifestation of behavioral indices of learning and memory impairment. Phosphopeptide enrichment with triple isotopic dimethylation labeling combined with online multidimensional separation and MS was used to profile phosphoproteome changes in 2- and 6-month-old TgCRND8 mice and congenic littermate controls. We identified 1026 phosphopeptides representing 1168 phosphorylation sites from 476 unique proteins. Of these, 595 phosphopeptides from 293 unique proteins were reliably quantified and 139 phosphopeptides were found to change significantly in the hippocampus of TgCRND8 mice following conversion from a presymptomatic to a symptomatic state.
语种英语
WOS记录号WOS:000317684800007
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/137475
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian, Peoples R China
2.Univ Ottawa, Ottawa Inst Syst Biol, Ottawa, ON K1H 8M5, Canada
3.Univ Ottawa, Neural Regenerat Lab, Dept Biochem Microbiol & Immunol, Ottawa, ON K1H 8M5, Canada
推荐引用方式
GB/T 7714
Wang, Fangjun,Blanchard, Alexandre P.,Elisma, Fred,et al. Phosphoproteome analysis of an early onset mouse model (TgCRND8) of Alzheimer's disease reveals temporal changes in neuronal and glia signaling pathways[J]. PROTEOMICS,2013,13(8):1292-1305.
APA Wang, Fangjun.,Blanchard, Alexandre P..,Elisma, Fred.,Granger, Matthew.,Xu, Hongbin.,...&Zou, Hanfa.(2013).Phosphoproteome analysis of an early onset mouse model (TgCRND8) of Alzheimer's disease reveals temporal changes in neuronal and glia signaling pathways.PROTEOMICS,13(8),1292-1305.
MLA Wang, Fangjun,et al."Phosphoproteome analysis of an early onset mouse model (TgCRND8) of Alzheimer's disease reveals temporal changes in neuronal and glia signaling pathways".PROTEOMICS 13.8(2013):1292-1305.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Fangjun]的文章
[Blanchard, Alexandre P.]的文章
[Elisma, Fred]的文章
百度学术
百度学术中相似的文章
[Wang, Fangjun]的文章
[Blanchard, Alexandre P.]的文章
[Elisma, Fred]的文章
必应学术
必应学术中相似的文章
[Wang, Fangjun]的文章
[Blanchard, Alexandre P.]的文章
[Elisma, Fred]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。