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Phosphoproteome analysis of an early onset mouse model (TgCRND8) of Alzheimer's disease reveals temporal changes in neuronal and glia signaling pathways
Wang, Fangjun1,2; Blanchard, Alexandre P.2,3; Elisma, Fred2; Granger, Matthew2,3; Xu, Hongbin2,3; Bennett, Steffany A. L.2,3; Figeys, Daniel2; Zou, Hanfa1
KeywordAlzheimer's Disease Animal Model Animal Proteomics Ms Phosphoproteomics Tgcrnd8
Source PublicationPROTEOMICS
2013-04-01
DOI10.1002/pmic.201200415
Volume13Issue:8Pages:1292-1305
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordRESPONSE MEDIATOR PROTEINS ; AMYLOID-BETA-PROTEIN ; MYELIN BASIC-PROTEIN ; IN-VIVO ; QUANTITATIVE PROTEOMICS ; TAU ; PHOSPHORYLATION ; AGGREGATION ; BINDING ; MICE
AbstractSustained exposure to soluble amyloid (A42) oligomers is predicted to impair synaptic function in the hippocampal-entorhinal circuit, signaling synaptic loss and precipitating cognitive impairment in Alzheimer's disease. Regional changes in overall patterns of protein phosphorylation are likely crucial to promote transition from a presymptomatic to a symptomatic state in response to accumulating A42. Here, we used unbiased proteomic approaches to compare the phosphoproteome of presymptomatic and symptomatic TgCRND8 mice and identify network disruptions in signaling pathways implicated in the manifestation of behavioral indices of learning and memory impairment. Phosphopeptide enrichment with triple isotopic dimethylation labeling combined with online multidimensional separation and MS was used to profile phosphoproteome changes in 2- and 6-month-old TgCRND8 mice and congenic littermate controls. We identified 1026 phosphopeptides representing 1168 phosphorylation sites from 476 unique proteins. Of these, 595 phosphopeptides from 293 unique proteins were reliably quantified and 139 phosphopeptides were found to change significantly in the hippocampus of TgCRND8 mice following conversion from a presymptomatic to a symptomatic state.
Language英语
WOS IDWOS:000317684800007
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/137475
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian, Peoples R China
2.Univ Ottawa, Ottawa Inst Syst Biol, Ottawa, ON K1H 8M5, Canada
3.Univ Ottawa, Neural Regenerat Lab, Dept Biochem Microbiol & Immunol, Ottawa, ON K1H 8M5, Canada
Recommended Citation
GB/T 7714
Wang, Fangjun,Blanchard, Alexandre P.,Elisma, Fred,et al. Phosphoproteome analysis of an early onset mouse model (TgCRND8) of Alzheimer's disease reveals temporal changes in neuronal and glia signaling pathways[J]. PROTEOMICS,2013,13(8):1292-1305.
APA Wang, Fangjun.,Blanchard, Alexandre P..,Elisma, Fred.,Granger, Matthew.,Xu, Hongbin.,...&Zou, Hanfa.(2013).Phosphoproteome analysis of an early onset mouse model (TgCRND8) of Alzheimer's disease reveals temporal changes in neuronal and glia signaling pathways.PROTEOMICS,13(8),1292-1305.
MLA Wang, Fangjun,et al."Phosphoproteome analysis of an early onset mouse model (TgCRND8) of Alzheimer's disease reveals temporal changes in neuronal and glia signaling pathways".PROTEOMICS 13.8(2013):1292-1305.
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