中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Crotonaldehyde induces apoptosis in alveolar macrophages through intracellular calcium, mitochondria and p53 signaling pathways
作者: Yang, Bi-cheng1, 2;  Pan, Xiu-jie3;  Yang, Zhi-hua3;  Xiao, Feng-jun3;  Liu, Xing-yu4;  Zhu, Mao-xiang3;  Xie, Jian-ping2
关键词: Crotonaldehyde ;  Apoptosis ;  ROS ;  Calcium ;  Mitochondria ;  p53
刊名: JOURNAL OF TOXICOLOGICAL SCIENCES
发表日期: 2013-04-01
卷: 38, 期:2, 页:225-235
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
英文摘要: Crotonaldehyde, a highly electrophilic alpha, beta-unsaturated aldehyde, is a ubiquitous environmental pollutant and a risk factor for multiple respiratory diseases. Crotonaldehyde is highly volatile and hydrophilic, so it is efficiently absorbed in the respiratory tract. Alveolar macrophages are major effector cells of the nonspecific host defence in the lung. The aim of this study was to investigate the molecular mechanisms and signaling pathways responsible for cell death of alveolar macrophage induced by crotonaldehyde. Our results show that crotonaldehyde induces apoptosis in alveolar macrophages, as indicated by phosphatidylserine externalization and DNA fragmentation. Pretreatment of alveolar macrophages with N-acetylcysteine, ascorbic acid, alpha-tocopherol, superoxide dismutase inhibited crotonaldehyde-induced apoptosis. Crotonaldehyde-induced apoptosis was characterized by ROS generation, GSH depletion, loss of mitochondrial membrane potential (Delta Psi m), the release of cytochrome c from mitochondria, caspase-3/7 and caspase-9 activation, elevation of intracellular Ca2+ concentration and the increase of p53 expression. Furthermore, pretreatment with either p53 inhibitor pifithrin-alpha or calcium chelator BAP-TA-AM effectively attenuated apoptosis induced by crotonaldehyde. Taken together, our results showed that crotonaldehyde induce apoptosis in alveolar macrophages through intracellular calcium, mitochondria and p53 signaling pathways. These results would help to illustrate the mechanism of toxicity induced by crotonaldehyde and to look for a novel treatment for diseases induced by exposure to crotonaldehyde-rich pollutants such as cigarette smoke.
关键词[WOS]: BRONCHIAL EPITHELIAL-CELLS ;  CIGARETTE-SMOKE ;  OXIDATIVE STRESS ;  GAS-CHROMATOGRAPHY ;  MASS-SPECTROMETRY ;  SHUTTLE VECTOR ;  ALDEHYDES ;  RELEASE ;  DEATH ;  MICE
语种: 英语
WOS记录号: WOS:000317163900006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137476
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
2.CNTC, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Henan, Peoples R China
3.Beijing Inst Radiat Med, Dept Radiat Toxicol & Oncol, Beijing 100850, Peoples R China
4.Shanghai Tobacco Corp, Ctr Technol, Beijing Work Stn, Beijing 101121, Peoples R China

Recommended Citation:
Yang, Bi-cheng,Pan, Xiu-jie,Yang, Zhi-hua,et al. Crotonaldehyde induces apoptosis in alveolar macrophages through intracellular calcium, mitochondria and p53 signaling pathways[J]. JOURNAL OF TOXICOLOGICAL SCIENCES,2013,38(2):225-235.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Yang, Bi-cheng]'s Articles
 [Pan, Xiu-jie]'s Articles
 [Yang, Zhi-hua]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Yang, Bi-cheng]‘s Articles
 [Pan, Xiu-jie]‘s Articles
 [Yang, Zhi-hua]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace