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Crotonaldehyde induces apoptosis in alveolar macrophages through intracellular calcium, mitochondria and p53 signaling pathways
Yang, Bi-cheng1,2; Pan, Xiu-jie3; Yang, Zhi-hua3; Xiao, Feng-jun3; Liu, Xing-yu4; Zhu, Mao-xiang3; Xie, Jian-ping2
关键词Crotonaldehyde Apoptosis Ros Calcium Mitochondria P53
刊名JOURNAL OF TOXICOLOGICAL SCIENCES
2013-04-01
38期:2页:225-235
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Toxicology
研究领域[WOS]Toxicology
关键词[WOS]BRONCHIAL EPITHELIAL-CELLS ; CIGARETTE-SMOKE ; OXIDATIVE STRESS ; GAS-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; SHUTTLE VECTOR ; ALDEHYDES ; RELEASE ; DEATH ; MICE
英文摘要Crotonaldehyde, a highly electrophilic alpha, beta-unsaturated aldehyde, is a ubiquitous environmental pollutant and a risk factor for multiple respiratory diseases. Crotonaldehyde is highly volatile and hydrophilic, so it is efficiently absorbed in the respiratory tract. Alveolar macrophages are major effector cells of the nonspecific host defence in the lung. The aim of this study was to investigate the molecular mechanisms and signaling pathways responsible for cell death of alveolar macrophage induced by crotonaldehyde. Our results show that crotonaldehyde induces apoptosis in alveolar macrophages, as indicated by phosphatidylserine externalization and DNA fragmentation. Pretreatment of alveolar macrophages with N-acetylcysteine, ascorbic acid, alpha-tocopherol, superoxide dismutase inhibited crotonaldehyde-induced apoptosis. Crotonaldehyde-induced apoptosis was characterized by ROS generation, GSH depletion, loss of mitochondrial membrane potential (Delta Psi m), the release of cytochrome c from mitochondria, caspase-3/7 and caspase-9 activation, elevation of intracellular Ca2+ concentration and the increase of p53 expression. Furthermore, pretreatment with either p53 inhibitor pifithrin-alpha or calcium chelator BAP-TA-AM effectively attenuated apoptosis induced by crotonaldehyde. Taken together, our results showed that crotonaldehyde induce apoptosis in alveolar macrophages through intracellular calcium, mitochondria and p53 signaling pathways. These results would help to illustrate the mechanism of toxicity induced by crotonaldehyde and to look for a novel treatment for diseases induced by exposure to crotonaldehyde-rich pollutants such as cigarette smoke.
语种英语
WOS记录号WOS:000317163900006
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/137476
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
2.CNTC, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Henan, Peoples R China
3.Beijing Inst Radiat Med, Dept Radiat Toxicol & Oncol, Beijing 100850, Peoples R China
4.Shanghai Tobacco Corp, Ctr Technol, Beijing Work Stn, Beijing 101121, Peoples R China
推荐引用方式
GB/T 7714
Yang, Bi-cheng,Pan, Xiu-jie,Yang, Zhi-hua,et al. Crotonaldehyde induces apoptosis in alveolar macrophages through intracellular calcium, mitochondria and p53 signaling pathways[J]. JOURNAL OF TOXICOLOGICAL SCIENCES,2013,38(2):225-235.
APA Yang, Bi-cheng.,Pan, Xiu-jie.,Yang, Zhi-hua.,Xiao, Feng-jun.,Liu, Xing-yu.,...&Xie, Jian-ping.(2013).Crotonaldehyde induces apoptosis in alveolar macrophages through intracellular calcium, mitochondria and p53 signaling pathways.JOURNAL OF TOXICOLOGICAL SCIENCES,38(2),225-235.
MLA Yang, Bi-cheng,et al."Crotonaldehyde induces apoptosis in alveolar macrophages through intracellular calcium, mitochondria and p53 signaling pathways".JOURNAL OF TOXICOLOGICAL SCIENCES 38.2(2013):225-235.
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