DICP OpenIR
Crotonaldehyde induces apoptosis in alveolar macrophages through intracellular calcium, mitochondria and p53 signaling pathways
Yang, Bi-cheng1,2; Pan, Xiu-jie3; Yang, Zhi-hua3; Xiao, Feng-jun3; Liu, Xing-yu4; Zhu, Mao-xiang3; Xie, Jian-ping2
KeywordCrotonaldehyde Apoptosis Ros Calcium Mitochondria P53
Source PublicationJOURNAL OF TOXICOLOGICAL SCIENCES
2013-04-01
Volume38Issue:2Pages:225-235
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectToxicology
WOS Research AreaToxicology
WOS KeywordBRONCHIAL EPITHELIAL-CELLS ; CIGARETTE-SMOKE ; OXIDATIVE STRESS ; GAS-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; SHUTTLE VECTOR ; ALDEHYDES ; RELEASE ; DEATH ; MICE
AbstractCrotonaldehyde, a highly electrophilic alpha, beta-unsaturated aldehyde, is a ubiquitous environmental pollutant and a risk factor for multiple respiratory diseases. Crotonaldehyde is highly volatile and hydrophilic, so it is efficiently absorbed in the respiratory tract. Alveolar macrophages are major effector cells of the nonspecific host defence in the lung. The aim of this study was to investigate the molecular mechanisms and signaling pathways responsible for cell death of alveolar macrophage induced by crotonaldehyde. Our results show that crotonaldehyde induces apoptosis in alveolar macrophages, as indicated by phosphatidylserine externalization and DNA fragmentation. Pretreatment of alveolar macrophages with N-acetylcysteine, ascorbic acid, alpha-tocopherol, superoxide dismutase inhibited crotonaldehyde-induced apoptosis. Crotonaldehyde-induced apoptosis was characterized by ROS generation, GSH depletion, loss of mitochondrial membrane potential (Delta Psi m), the release of cytochrome c from mitochondria, caspase-3/7 and caspase-9 activation, elevation of intracellular Ca2+ concentration and the increase of p53 expression. Furthermore, pretreatment with either p53 inhibitor pifithrin-alpha or calcium chelator BAP-TA-AM effectively attenuated apoptosis induced by crotonaldehyde. Taken together, our results showed that crotonaldehyde induce apoptosis in alveolar macrophages through intracellular calcium, mitochondria and p53 signaling pathways. These results would help to illustrate the mechanism of toxicity induced by crotonaldehyde and to look for a novel treatment for diseases induced by exposure to crotonaldehyde-rich pollutants such as cigarette smoke.
Language英语
WOS IDWOS:000317163900006
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/137476
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
2.CNTC, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Henan, Peoples R China
3.Beijing Inst Radiat Med, Dept Radiat Toxicol & Oncol, Beijing 100850, Peoples R China
4.Shanghai Tobacco Corp, Ctr Technol, Beijing Work Stn, Beijing 101121, Peoples R China
Recommended Citation
GB/T 7714
Yang, Bi-cheng,Pan, Xiu-jie,Yang, Zhi-hua,et al. Crotonaldehyde induces apoptosis in alveolar macrophages through intracellular calcium, mitochondria and p53 signaling pathways[J]. JOURNAL OF TOXICOLOGICAL SCIENCES,2013,38(2):225-235.
APA Yang, Bi-cheng.,Pan, Xiu-jie.,Yang, Zhi-hua.,Xiao, Feng-jun.,Liu, Xing-yu.,...&Xie, Jian-ping.(2013).Crotonaldehyde induces apoptosis in alveolar macrophages through intracellular calcium, mitochondria and p53 signaling pathways.JOURNAL OF TOXICOLOGICAL SCIENCES,38(2),225-235.
MLA Yang, Bi-cheng,et al."Crotonaldehyde induces apoptosis in alveolar macrophages through intracellular calcium, mitochondria and p53 signaling pathways".JOURNAL OF TOXICOLOGICAL SCIENCES 38.2(2013):225-235.
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