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题名: Enantioseparations of amino acids by capillary array electrophoresis with 532 nm laser induced fluorescence detection
作者: Liu, Kaiying1;  Wang, Li2
关键词: Capillary array electrophoresis ;  Chiral amino acids ;  Cyclodextrins ;  Enantiomeric excess ;  Rhodamine-core fluorescent probe
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2013-06-21
DOI: 10.1016/j.chroma.2013.04.058
卷: 1295, 页:142-146
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Capillary array electrophoresis (CAE) is a promising technique for multiple enantiomeric separations. Carboxytetramethylrhodamine succinimidyl ester (TAMRA SE), a rhodamine-core fluorescent probe, has rarely been applied as an original precolumn derivatization reagent for chiral amino acid (AA) analysis so far. For these purposes, high-throughput enantiomeric separations of 12 TAMRA SE-AAs by a homemade 532 nm CAE-LIF scanner are presented. The effect of cyclodextrins (CDs) and a variety of organic modifiers was quickly investigated. Baseline separations were achieved in 100 mM Tris-borate buffer (pH 10.0) containing 2 mM beta-CD and 10 mM hexamethylenediamine (HDA). Multiple determination of the enantiomeric excess (ee) in non-racemic mixtures of alanine is successfully presented. (C) 2013 Elsevier B.V. All rights reserved.
关键词[WOS]: MICELLAR ELECTROKINETIC CHROMATOGRAPHY ;  LIF DETECTION ;  CHIRAL SEPARATION ;  SELECTORS ;  PEPTIDES ;  REAGENTS ;  CE
语种: 英语
WOS记录号: WOS:000320294000015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137479
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Maritime Univ, Dept Phys, Inst Environm Engn, Dalian 116026, Peoples R China
2.Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China

Recommended Citation:
Liu, Kaiying,Wang, Li. Enantioseparations of amino acids by capillary array electrophoresis with 532 nm laser induced fluorescence detection[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1295:142-146.
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