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Enantioseparations of amino acids by capillary array electrophoresis with 532 nm laser induced fluorescence detection
Liu, Kaiying1; Wang, Li2
KeywordCapillary Array Electrophoresis Chiral Amino Acids Cyclodextrins Enantiomeric Excess Rhodamine-core Fluorescent Probe
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2013-06-21
DOI10.1016/j.chroma.2013.04.058
Volume1295Pages:142-146
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordMICELLAR ELECTROKINETIC CHROMATOGRAPHY ; LIF DETECTION ; CHIRAL SEPARATION ; SELECTORS ; PEPTIDES ; REAGENTS ; CE
AbstractCapillary array electrophoresis (CAE) is a promising technique for multiple enantiomeric separations. Carboxytetramethylrhodamine succinimidyl ester (TAMRA SE), a rhodamine-core fluorescent probe, has rarely been applied as an original precolumn derivatization reagent for chiral amino acid (AA) analysis so far. For these purposes, high-throughput enantiomeric separations of 12 TAMRA SE-AAs by a homemade 532 nm CAE-LIF scanner are presented. The effect of cyclodextrins (CDs) and a variety of organic modifiers was quickly investigated. Baseline separations were achieved in 100 mM Tris-borate buffer (pH 10.0) containing 2 mM beta-CD and 10 mM hexamethylenediamine (HDA). Multiple determination of the enantiomeric excess (ee) in non-racemic mixtures of alanine is successfully presented. (C) 2013 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000320294000015
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/137479
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Maritime Univ, Dept Phys, Inst Environm Engn, Dalian 116026, Peoples R China
2.Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Liu, Kaiying,Wang, Li. Enantioseparations of amino acids by capillary array electrophoresis with 532 nm laser induced fluorescence detection[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1295:142-146.
APA Liu, Kaiying,&Wang, Li.(2013).Enantioseparations of amino acids by capillary array electrophoresis with 532 nm laser induced fluorescence detection.JOURNAL OF CHROMATOGRAPHY A,1295,142-146.
MLA Liu, Kaiying,et al."Enantioseparations of amino acids by capillary array electrophoresis with 532 nm laser induced fluorescence detection".JOURNAL OF CHROMATOGRAPHY A 1295(2013):142-146.
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