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Novel Hybrid Structure Silica/CdTe/Molecularly Imprinted Polymer: Synthesis, Specific Recognition, and Quantitative Fluorescence Detection of Bovine Hemoglobin
Li, Dong-Yan1,2,3; He, Xi-Wen1,2,3; Chen, Yang1,2,3; Li, Wen-You1,2,3; Zhang, Yu-Kui1,2,3,4
关键词Molecularly Imprinted Polymer Quantum Dots Fluorescent Detection Protein Recognition
刊名ACS APPLIED MATERIALS & INTERFACES
2013-12-11
DOI10.1021/am403942y
5期:23页:12609-12616
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Technology
类目[WOS]Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
研究领域[WOS]Science & Technology - Other Topics ; Materials Science
关键词[WOS]CDTE QUANTUM DOTS ; SILICA NANOPARTICLES ; PROTEIN ; SURFACE ; NANOCRYSTALS ; BIOAPPLICATIONS ; HYDROLYSIS
英文摘要This work presented a novel strategy for the synthesis of the hybrid structure silica/CdTe/molecularly imprinted polymer (Si-NP/CdTe/MIP) to recognize and detect the template bovine hemoglobin (BHb). First, amino-functionalized silica nanoparticles (Si-NP) and carboxyl-terminated CdTe quantum dots (QDs) were assembled into composite nanoparticles (Si-NP/CdTe) using the EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) chemistry. Next, Si-NP/CdTe/MIP was synthesized by anchoring molecularly imprinted polymer (MIP) layer on the surface of Si-NP/CdTe through the sol-gel technique and surface imprinting technique. The hybrid structure possessed the selectivity of molecular imprinting technique and the sensitivity of CdTe QDs as well as well-defined morphology. The binding experiment and fluorescence method demonstrated its special recognition performance toward the template BHb. Under the optimized conditions, the fluorescence intensity of the Si-NP/CdTe/MIP decreased linearly with the increase of BHb in the concentration range 0.02-2.1 mu M, and the detection limit was 9.4 nM. Moreover, the reusability and reproducibility and the successful applications in practical samples indicated the synthesis of Si-NP/CdTe/MIP provided an alternative solution for special recognition and determination of protein from real samples.
语种英语
WOS记录号WOS:000328439600051
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/137482
专题中国科学院大连化学物理研究所
作者单位1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
3.Synerget Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
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Li, Dong-Yan,He, Xi-Wen,Chen, Yang,et al. Novel Hybrid Structure Silica/CdTe/Molecularly Imprinted Polymer: Synthesis, Specific Recognition, and Quantitative Fluorescence Detection of Bovine Hemoglobin[J]. ACS APPLIED MATERIALS & INTERFACES,2013,5(23):12609-12616.
APA Li, Dong-Yan,He, Xi-Wen,Chen, Yang,Li, Wen-You,&Zhang, Yu-Kui.(2013).Novel Hybrid Structure Silica/CdTe/Molecularly Imprinted Polymer: Synthesis, Specific Recognition, and Quantitative Fluorescence Detection of Bovine Hemoglobin.ACS APPLIED MATERIALS & INTERFACES,5(23),12609-12616.
MLA Li, Dong-Yan,et al."Novel Hybrid Structure Silica/CdTe/Molecularly Imprinted Polymer: Synthesis, Specific Recognition, and Quantitative Fluorescence Detection of Bovine Hemoglobin".ACS APPLIED MATERIALS & INTERFACES 5.23(2013):12609-12616.
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