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Comparison of the salt-induced activation of Pseudomonas cepacia lipase in organic media by phosphate buffer and sodium sulfate
Jin Qianru; Jia Guoqing; Wang Xiuli; Li Can
关键词Crude Pseudomonas Cepacia Lipase Salt-induced Activation Of Enzyme Phosphate Buffer Sodium Sulfate Thermogravimetric Analysis Local Polarity Fluorescence Probe
刊名CHINESE JOURNAL OF CATALYSIS
2013-06-01
DOI10.1016/S1872-2067(12)60558-0
34期:6页:1224-1231
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Applied ; Chemistry, Physical ; Engineering, Chemical
研究领域[WOS]Chemistry ; Engineering
关键词[WOS]ENZYME-ACTIVITY ; SOLVENTS ; WATER ; BIOCATALYSIS ; PROTEIN ; ENANTIOSELECTIVITY
英文摘要The effects of a kosmotropic phosphate buffer (PB) or sodium sulfate (SS) on the enzymatic activity of crude Pseudomonas cepacia lipase (PCL) in organic media were explored. Previously, it was believed that SS acts as an effective enzyme activator, and PB was only used to adjust the solution pH. The present work showed PB-induced activation of PCL in the transesterification activity, where an increase of nearly 10-fold was achieved by carefully controlling the lyophilization condition. This activation, which gave about one-half of the native catalytic efficiency of PCL in aqueous media, was even more effective than the SS-induced activation. Thermogravimetric analysis of PCL preparations showed that different local environments surrounded PCL due to the presence of PB or SS. The local polarity of PCL preparations suspended in n-hexane was investigated by fluorescence spectroscopy using 2-(4'-amino-2'-hydroxy phenyl)benzoxazole as the probe. SS-incorporated PCL showed a much stronger local polarity than PB-incorporated and salt-free PCL. A correlation was found between the transesterification activity and local polarity of the enzyme preparation as a function of salt content The data confirmed that the salt-induced activation of PCL in organic media can be attributed in part to the polar environment constituted by the salt-bonded water surrounding the PCL. (c) 2013, Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000320747400022
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/137497
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
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Jin Qianru,Jia Guoqing,Wang Xiuli,et al. Comparison of the salt-induced activation of Pseudomonas cepacia lipase in organic media by phosphate buffer and sodium sulfate[J]. CHINESE JOURNAL OF CATALYSIS,2013,34(6):1224-1231.
APA Jin Qianru,Jia Guoqing,Wang Xiuli,&Li Can.(2013).Comparison of the salt-induced activation of Pseudomonas cepacia lipase in organic media by phosphate buffer and sodium sulfate.CHINESE JOURNAL OF CATALYSIS,34(6),1224-1231.
MLA Jin Qianru,et al."Comparison of the salt-induced activation of Pseudomonas cepacia lipase in organic media by phosphate buffer and sodium sulfate".CHINESE JOURNAL OF CATALYSIS 34.6(2013):1224-1231.
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