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题名: Comparison of the salt-induced activation of Pseudomonas cepacia lipase in organic media by phosphate buffer and sodium sulfate
作者: Jin Qianru1;  Jia Guoqing1;  Wang Xiuli1;  Li Can1
关键词: Crude Pseudomonas cepacia lipase ;  Salt-induced activation of enzyme ;  Phosphate buffer ;  Sodium sulfate ;  Thermogravimetric analysis ;  Local polarity ;  Fluorescence probe
刊名: CHINESE JOURNAL OF CATALYSIS
发表日期: 2013-06-01
DOI: 10.1016/S1872-2067(12)60558-0
卷: 34, 期:6, 页:1224-1231
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Applied ;  Chemistry, Physical ;  Engineering, Chemical
研究领域[WOS]: Chemistry ;  Engineering
英文摘要: The effects of a kosmotropic phosphate buffer (PB) or sodium sulfate (SS) on the enzymatic activity of crude Pseudomonas cepacia lipase (PCL) in organic media were explored. Previously, it was believed that SS acts as an effective enzyme activator, and PB was only used to adjust the solution pH. The present work showed PB-induced activation of PCL in the transesterification activity, where an increase of nearly 10-fold was achieved by carefully controlling the lyophilization condition. This activation, which gave about one-half of the native catalytic efficiency of PCL in aqueous media, was even more effective than the SS-induced activation. Thermogravimetric analysis of PCL preparations showed that different local environments surrounded PCL due to the presence of PB or SS. The local polarity of PCL preparations suspended in n-hexane was investigated by fluorescence spectroscopy using 2-(4'-amino-2'-hydroxy phenyl)benzoxazole as the probe. SS-incorporated PCL showed a much stronger local polarity than PB-incorporated and salt-free PCL. A correlation was found between the transesterification activity and local polarity of the enzyme preparation as a function of salt content The data confirmed that the salt-induced activation of PCL in organic media can be attributed in part to the polar environment constituted by the salt-bonded water surrounding the PCL. (c) 2013, Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.
关键词[WOS]: ENZYME-ACTIVITY ;  SOLVENTS ;  WATER ;  BIOCATALYSIS ;  PROTEIN ;  ENANTIOSELECTIVITY
语种: 英语
WOS记录号: WOS:000320747400022
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137497
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China

Recommended Citation:
Jin Qianru,Jia Guoqing,Wang Xiuli,et al. Comparison of the salt-induced activation of Pseudomonas cepacia lipase in organic media by phosphate buffer and sodium sulfate[J]. CHINESE JOURNAL OF CATALYSIS,2013,34(6):1224-1231.
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