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Bienzymatic glucose biosensor based on direct electrochemistry of cytochrome c on gold nanoparticles/polyaniline nanospheres composite
Xiang, Cuili1; Zou, Yongjin1; Qiu, Shujun1; Sun, Lixian1,2; Xu, Fen1,3; Zhou, Huaiying1
KeywordCytochrome c Gold Nanoparticles Polyaniline Nanoshpere Direct Electron Transfer Glucose Biosensor
Source PublicationTALANTA
2013-06-15
DOI10.1016/j.talanta.2013.02.022
Volume110Pages:96-100
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordGLASSY-CARBON ELECTRODE ; PLATINUM NANOPARTICLES ; HYDROGEN-PEROXIDE ; CHITOSAN ; FILM ; ELECTROCATALYSIS ; NANOTUBES ; PROTEINS ; IMMOBILIZATION ; OXIDASE
AbstractGold nanoparticles (GNPs) assembled polyaniline nanospheres (PANS) composite were prepared by noncovalent strategy. Cytochrome c (Cyt c) was electrostatically adsorbed on the GNPs/PANS modified electrode. The direct electron transfer (DET) between Cyt c and GNPs/PANS modified electrode was investigated. Cyt c shows a couple of quasi-reversible and well-defined cyclic voltammetry (CV) peaks with a formal potential (E-0) of -0.27 V (versus Ag/AgCl) in pH 7.0 phosphate buffer solution (PBS). The Cyt c/GNPs/PANS modified electrode gives an improved electrocatalytic activity toward the reduction of hydrogen peroxide (H2O2). The catalysis currents increase linearly to the H2O2 concentration in a wide range of 0.01-2.4 mM with a correlation coefficient 0.998. After immobilized glucose oxidase (GOD) on the Cyt c/GNPs/PANS modified electrode by Nafion polymer, a bienzymatic glucose biosensor was further prepared for sensitive detection of glucose. The amperometric detection of glucose was assayed by potentiostating the bienzymatic electrode at -0.2 V versus Ag/AgCl to reduce the enzymatically produced H2O2 with minimal interference from the coexisting electroactive compounds. The proposed glucose biosensor exhibits good response performance to glucose with a wide linear range from 0.01 to 3.2 mM with a correlation coefficient of 0.997. The electrode has high sensitivity of 63.1 [mu A mM(-1) cm(-2) and a detection limit 0.01 mM at the signal-to-noise ratio of 3. Moreover, the glucose biosensor possesses good stability and reproducibility. (C) 2013 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000319089500015
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/137500
Collection中国科学院大连化学物理研究所
Affiliation1.Guilin Univ Elect Technol, Guangxi Key Lab Informat Mat, Guilin 541004, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Liaoning Normal Univ, Dept Chem & Chem Engn, Dalian 116029, Peoples R China
Recommended Citation
GB/T 7714
Xiang, Cuili,Zou, Yongjin,Qiu, Shujun,et al. Bienzymatic glucose biosensor based on direct electrochemistry of cytochrome c on gold nanoparticles/polyaniline nanospheres composite[J]. TALANTA,2013,110:96-100.
APA Xiang, Cuili,Zou, Yongjin,Qiu, Shujun,Sun, Lixian,Xu, Fen,&Zhou, Huaiying.(2013).Bienzymatic glucose biosensor based on direct electrochemistry of cytochrome c on gold nanoparticles/polyaniline nanospheres composite.TALANTA,110,96-100.
MLA Xiang, Cuili,et al."Bienzymatic glucose biosensor based on direct electrochemistry of cytochrome c on gold nanoparticles/polyaniline nanospheres composite".TALANTA 110(2013):96-100.
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