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题名: Bienzymatic glucose biosensor based on direct electrochemistry of cytochrome c on gold nanoparticles/polyaniline nanospheres composite
作者: Xiang, Cuili1;  Zou, Yongjin1;  Qiu, Shujun1;  Sun, Lixian1, 2;  Xu, Fen1, 3;  Zhou, Huaiying1
关键词: Cytochrome c ;  Gold nanoparticles ;  Polyaniline nanoshpere ;  Direct electron transfer ;  Glucose biosensor
刊名: TALANTA
发表日期: 2013-06-15
DOI: 10.1016/j.talanta.2013.02.022
卷: 110, 页:96-100
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Gold nanoparticles (GNPs) assembled polyaniline nanospheres (PANS) composite were prepared by noncovalent strategy. Cytochrome c (Cyt c) was electrostatically adsorbed on the GNPs/PANS modified electrode. The direct electron transfer (DET) between Cyt c and GNPs/PANS modified electrode was investigated. Cyt c shows a couple of quasi-reversible and well-defined cyclic voltammetry (CV) peaks with a formal potential (E-0) of -0.27 V (versus Ag/AgCl) in pH 7.0 phosphate buffer solution (PBS). The Cyt c/GNPs/PANS modified electrode gives an improved electrocatalytic activity toward the reduction of hydrogen peroxide (H2O2). The catalysis currents increase linearly to the H2O2 concentration in a wide range of 0.01-2.4 mM with a correlation coefficient 0.998. After immobilized glucose oxidase (GOD) on the Cyt c/GNPs/PANS modified electrode by Nafion polymer, a bienzymatic glucose biosensor was further prepared for sensitive detection of glucose. The amperometric detection of glucose was assayed by potentiostating the bienzymatic electrode at -0.2 V versus Ag/AgCl to reduce the enzymatically produced H2O2 with minimal interference from the coexisting electroactive compounds. The proposed glucose biosensor exhibits good response performance to glucose with a wide linear range from 0.01 to 3.2 mM with a correlation coefficient of 0.997. The electrode has high sensitivity of 63.1 [mu A mM(-1) cm(-2) and a detection limit 0.01 mM at the signal-to-noise ratio of 3. Moreover, the glucose biosensor possesses good stability and reproducibility. (C) 2013 Elsevier B.V. All rights reserved.
关键词[WOS]: GLASSY-CARBON ELECTRODE ;  PLATINUM NANOPARTICLES ;  HYDROGEN-PEROXIDE ;  CHITOSAN ;  FILM ;  ELECTROCATALYSIS ;  NANOTUBES ;  PROTEINS ;  IMMOBILIZATION ;  OXIDASE
语种: 英语
WOS记录号: WOS:000319089500015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137500
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Guilin Univ Elect Technol, Guangxi Key Lab Informat Mat, Guilin 541004, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Liaoning Normal Univ, Dept Chem & Chem Engn, Dalian 116029, Peoples R China

Recommended Citation:
Xiang, Cuili,Zou, Yongjin,Qiu, Shujun,et al. Bienzymatic glucose biosensor based on direct electrochemistry of cytochrome c on gold nanoparticles/polyaniline nanospheres composite[J]. TALANTA,2013,110:96-100.
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