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Preparation of a butyl-silica hybrid monolithic column with a "one-pot" process for bioseparation by capillary liquid chromatography
Zhang, Zhenbin1,2; Wang, Fangjun1; Ou, Junjie1; Lin, Hui1,2; Dong, Jing1; Zou, Hanfa1
关键词One Pot Sol-gel Butyl-silica Hybrid Monolith Capillary Liquid Chromatography Bioseparation
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
2013-03-01
DOI10.1007/s00216-012-6589-z
405期:7页:2265-2271
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]SEPARATION ; ELECTROCHROMATOGRAPHY ; PROTEINS ; FLOW
英文摘要A butyl-silica hybrid monolithic column for bioseparation by capillary liquid chromatography (cLC) was prepared with butyl methacrylate and alkoxysilanes through a "one-pot" process. The effects of polycondensation temperature, volume percentage of N,N'-dimethylformamide, and content of cetyltrimethylammonium bromide and butyl methacrylate on the morphologies of the hybrid monolithic columns prepared were investigated in detail. Baseline separations of proteins and small peptides on the hybrid monolithic column were achieved by cLC with gradient elution. In addition, the resulting hybrid column was also applied for analysis of tryptic digests of bovine serum albumin by cLC coupled with tandem mass spectrometry. The results demonstrate its potential application in separation of complex biological samples.
语种英语
WOS记录号WOS:000315043800016
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/137531
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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GB/T 7714
Zhang, Zhenbin,Wang, Fangjun,Ou, Junjie,et al. Preparation of a butyl-silica hybrid monolithic column with a "one-pot" process for bioseparation by capillary liquid chromatography[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2013,405(7):2265-2271.
APA Zhang, Zhenbin,Wang, Fangjun,Ou, Junjie,Lin, Hui,Dong, Jing,&Zou, Hanfa.(2013).Preparation of a butyl-silica hybrid monolithic column with a "one-pot" process for bioseparation by capillary liquid chromatography.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,405(7),2265-2271.
MLA Zhang, Zhenbin,et al."Preparation of a butyl-silica hybrid monolithic column with a "one-pot" process for bioseparation by capillary liquid chromatography".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 405.7(2013):2265-2271.
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