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题名: Preparation of a butyl-silica hybrid monolithic column with a "one-pot" process for bioseparation by capillary liquid chromatography
作者: Zhang, Zhenbin1, 2;  Wang, Fangjun1;  Ou, Junjie1;  Lin, Hui1, 2;  Dong, Jing1;  Zou, Hanfa1
关键词: One pot ;  Sol-gel ;  Butyl-silica hybrid monolith ;  Capillary liquid chromatography ;  Bioseparation
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2013-03-01
DOI: 10.1007/s00216-012-6589-z
卷: 405, 期:7, 页:2265-2271
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A butyl-silica hybrid monolithic column for bioseparation by capillary liquid chromatography (cLC) was prepared with butyl methacrylate and alkoxysilanes through a "one-pot" process. The effects of polycondensation temperature, volume percentage of N,N'-dimethylformamide, and content of cetyltrimethylammonium bromide and butyl methacrylate on the morphologies of the hybrid monolithic columns prepared were investigated in detail. Baseline separations of proteins and small peptides on the hybrid monolithic column were achieved by cLC with gradient elution. In addition, the resulting hybrid column was also applied for analysis of tryptic digests of bovine serum albumin by cLC coupled with tandem mass spectrometry. The results demonstrate its potential application in separation of complex biological samples.
关键词[WOS]: SEPARATION ;  ELECTROCHROMATOGRAPHY ;  PROTEINS ;  FLOW
语种: 英语
WOS记录号: WOS:000315043800016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137531
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Zhang, Zhenbin,Wang, Fangjun,Ou, Junjie,et al. Preparation of a butyl-silica hybrid monolithic column with a "one-pot" process for bioseparation by capillary liquid chromatography[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2013,405(7):2265-2271.
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