DICP OpenIR
Preparation of a butyl-silica hybrid monolithic column with a "one-pot" process for bioseparation by capillary liquid chromatography
Zhang, Zhenbin1,2; Wang, Fangjun1; Ou, Junjie1; Lin, Hui1,2; Dong, Jing1; Zou, Hanfa1
KeywordOne Pot Sol-gel Butyl-silica Hybrid Monolith Capillary Liquid Chromatography Bioseparation
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
2013-03-01
DOI10.1007/s00216-012-6589-z
Volume405Issue:7Pages:2265-2271
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordSEPARATION ; ELECTROCHROMATOGRAPHY ; PROTEINS ; FLOW
AbstractA butyl-silica hybrid monolithic column for bioseparation by capillary liquid chromatography (cLC) was prepared with butyl methacrylate and alkoxysilanes through a "one-pot" process. The effects of polycondensation temperature, volume percentage of N,N'-dimethylformamide, and content of cetyltrimethylammonium bromide and butyl methacrylate on the morphologies of the hybrid monolithic columns prepared were investigated in detail. Baseline separations of proteins and small peptides on the hybrid monolithic column were achieved by cLC with gradient elution. In addition, the resulting hybrid column was also applied for analysis of tryptic digests of bovine serum albumin by cLC coupled with tandem mass spectrometry. The results demonstrate its potential application in separation of complex biological samples.
Language英语
WOS IDWOS:000315043800016
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/137531
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Zhenbin,Wang, Fangjun,Ou, Junjie,et al. Preparation of a butyl-silica hybrid monolithic column with a "one-pot" process for bioseparation by capillary liquid chromatography[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2013,405(7):2265-2271.
APA Zhang, Zhenbin,Wang, Fangjun,Ou, Junjie,Lin, Hui,Dong, Jing,&Zou, Hanfa.(2013).Preparation of a butyl-silica hybrid monolithic column with a "one-pot" process for bioseparation by capillary liquid chromatography.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,405(7),2265-2271.
MLA Zhang, Zhenbin,et al."Preparation of a butyl-silica hybrid monolithic column with a "one-pot" process for bioseparation by capillary liquid chromatography".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 405.7(2013):2265-2271.
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