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题名: Purification of saponins from leaves of Panax notoginseng using preparative two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography
作者: Guo, Xiujie1;  Zhang, Xiuli1;  Feng, Jiatao1;  Guo, Zhimou1;  Xiao, Yuansheng1;  Liang, Xinmiao1
关键词: Saponins ;  Purification ;  Reversed-phase liquid chromatography ;  Hydrophilic interaction chromatography ;  Panax notoginseng (Burk.) FHChen
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2013-04-01
DOI: 10.1007/s00216-013-6721-8
卷: 405, 期:10, 页:3413-3421
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Saponins are widely distributed in the plant kingdom and have been shown to be active components of many medicinal herbs. In this study, a two-dimensional purification method based on reversed-phase liquid chromatography coupled with hydrophilic interaction liquid chromatography was successfully applied to purify saponins from leaves of Panax notoginseng. Nine saponin reference standards were used to test the separation modes and columns. The standards could not be resolved using C-18 columns owing to their limited polar selectivity. However, they were completely separated on a XAmide column in hydrophilic interaction liquid chromatography mode, including two pairs of standards that were coeluted on a C-18 column. The elution order of the standards on the two columns was sufficiently different, with a correlation coefficient between retention times on the C-18 and XAmide columns of 0.0126, indicating good column orthogonality. Therefore, the first-dimension preparation was performed on a C-18 column, followed by a XAmide column that was used to separate the fractions in the second dimension. Fifty-four fractions were prepared in the first dimension, with 25 fractions rich in saponins. Eight saponins, including two pairs of isomeric saponins and one new saponin, were isolated and identified from three representative fractions. This procedure was shown to be an effective approach for the preparative isolation and purification of saponins from leaves of P. notoginseng. Moreover, this method could possibly be employed in the purification of low-content and novel active saponins from natural products.
关键词[WOS]: HYDROPHILIC INTERACTION CHROMATOGRAPHY ;  COUNTER-CURRENT CHROMATOGRAPHY ;  FLIGHT MASS-SPECTROMETRY ;  DAMMARANE-SAPONINS ;  RETENTION BEHAVIOR ;  STATIONARY-PHASE ;  PLANT SAPONINS ;  HILIC-RP ;  GINSENG ;  SEPARATION
语种: 英语
WOS记录号: WOS:000316338700041
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137552
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Guo, Xiujie,Zhang, Xiuli,Feng, Jiatao,et al. Purification of saponins from leaves of Panax notoginseng using preparative two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2013,405(10):3413-3421.
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