DICP OpenIR
Determination of Steroid Hormones in Mouse Brain by Online Capillary Solid Phase Extraction-Nano Liquid Chromatography-Tandem Mass Spectrometry
Shi Xian-Zhe1; Cai Bin2; Huang Si-Teng3; Shan Yuan-Hong1; Zhang Ting-Ting2; Lu Xin1; Xu Guo-Wang1
KeywordNano Liquid Chromatography Tandem Mass Spectrometry Steroid Hormones Brain Tissue
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2013-04-01
DOI10.3724/SP.J.1096.2013.20660
Volume41Issue:4Pages:517-522
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordENDOGENOUS ESTROGENS ; NEUROSTEROIDS ; TISSUE ; PERFORMANCE ; SAMPLES
AbstractThe modulation of steroid hormones on brain is attracting more and more attention. However, the detection of steroid hormones is faced with great challenge owning to the very low content in brain tissue. In this work, the preparation conditions of steroid hormones extracted from brain tissue were optimized. Ethanol was the best extraction solvent for brain homogenization as it readily penetrated cell membranes and was a good solvent for steroid hormones. After purified by Oasis HLB SPE, the matrix effect was reduced significantly. An on-line capillary solid phase extraction -nano liquid chromatographic system was built based on valve-switching 75 mu m i. d. trap column and analytical column. Tandem mass spectrometer with NanoESI source was used as the detector for the determination of ten brain steroid hormones. The method showed good precision (intraday RSD 4. 5% -12. 8% and inter day RSD 8. 3% -20. 3%) and linearity (r>0. 995). The limits of detection in solution were from 20 ng/L to 10 mu g/L for 10 steroid hormones. The sensitivity of the method was gained in two orders of magnitude compared with that of conventional HPLC/MS when the equal amounts of the steroid hormones were injected. The recoveries were above 60% at three concentrations except for androsterone while the matrix effect was inapparent except for progesterone. Brain tissue samples from mouse were analyzed using the new method. Four steroids were identified and quantified including testosterone 0. 47 ng/g, corticosterone 26. 2 ng/g, aldosterone 0. 49 ng/g and hydrocortisone 0. 06 ng/g. The results showed the method has many advantages such as high sensitivity, low sample amount and solvent consumption which is suitable for the determination of steroid hormones in biological tissue.
Language英语
WOS IDWOS:000324165600008
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/137555
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.ThermoFisher Sci Inc China, Shanghai 201203, Peoples R China
3.Tianjin Univ, Sch Chem Engn & Technol, Tianjin 30019, Peoples R China
Recommended Citation
GB/T 7714
Shi Xian-Zhe,Cai Bin,Huang Si-Teng,et al. Determination of Steroid Hormones in Mouse Brain by Online Capillary Solid Phase Extraction-Nano Liquid Chromatography-Tandem Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2013,41(4):517-522.
APA Shi Xian-Zhe.,Cai Bin.,Huang Si-Teng.,Shan Yuan-Hong.,Zhang Ting-Ting.,...&Xu Guo-Wang.(2013).Determination of Steroid Hormones in Mouse Brain by Online Capillary Solid Phase Extraction-Nano Liquid Chromatography-Tandem Mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,41(4),517-522.
MLA Shi Xian-Zhe,et al."Determination of Steroid Hormones in Mouse Brain by Online Capillary Solid Phase Extraction-Nano Liquid Chromatography-Tandem Mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 41.4(2013):517-522.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Shi Xian-Zhe]'s Articles
[Cai Bin]'s Articles
[Huang Si-Teng]'s Articles
Baidu academic
Similar articles in Baidu academic
[Shi Xian-Zhe]'s Articles
[Cai Bin]'s Articles
[Huang Si-Teng]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Shi Xian-Zhe]'s Articles
[Cai Bin]'s Articles
[Huang Si-Teng]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.