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题名: Molecular cloning of partial 14-3-3 genes in the marine sponge Hymeniacidon perleve and its role in differentiating infectious and non-infectious bacteria
作者: Fu WanTao1, 4, 5;  Zhang JuLin1, 6;  Zheng ChangBo7;  Liu Jing1;  An ZhongFu1;  Liu HongWen8;  Zhang Wei2, 3, 4
关键词: marine sponge Hymeniacidon perleve ;  14-3-3 mRNA ;  RT-PCR ;  infectious bacteria
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2013-03-01
DOI: 10.1007/s11434-012-5400-z
卷: 58, 期:7, 页:766-776
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: Sponges (phylum Porifera) are the phylogenetically oldest metazoa and highly efficient filter feeders. In the marine ecosystem, they are unconditionally exposed to environmental stresses. Understanding the sponge-bacteria interaction is hence of both ecological and biological significance. This study investigated the specific interaction between the sponge Hymeniacidon perleve and the non-infectious bacteria, Escherichia coli and infectious bacteria, Vibrio spp. by measuring the 14-3-3 mRNA expression of H. perleve. Three partial cDNAs of 14-3-3 proteins and partial 18S RNA in H. perleve were cloned and sequenced. Using Reverse-transcription real-time PCR, the 14-3-3 mRNA expression of H. perleve was examined when exposed to three common bacteria in aquatic water-E. coli and two Vibrio spp. for different time and dosages. H. perleve could efficiently remove E. coli from the water column without self-infection; however Vibrio at higher dosages infected H. perleve. When H. perleve was exposed to E. coli (1.1-10(7) CFU mL(-1)), V. anguillarum II (1.2-10(6) CFU mL(-1)) and V. alginolyticus (3.6-10(5) CFU mL(-1)) for 6 h, the 14-3-3 mRNA expression in the V. anguillarum II and V. alginolyticus groups was down-regulated by 2.67- and 2.36-fold, respectively. The 14-3-3 mRNA expression in the E. coli group was not significantly different. However, no clear trend was observed on the 14-3-3 transcript levels of H. perleve in response to different doses of V. anguillarum II for different time. The results demonstrated that infectious bacteria can be discriminated by 14-3-3 mRNA expression of sponge H. perleve.
关键词[WOS]: ACTIVATED PROTEIN-KINASE ;  INNATE IMMUNE DEFENSE ;  SUBERITES-DOMUNCULA ;  AQUATIC ENVIRONMENTS ;  INDUCTION ;  EVOLUTION ;  APOPTOSIS ;  FOOD ;  14-3-3-PROTEINS ;  SPECIFICITY
语种: 英语
WOS记录号: WOS:000315446200010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137556
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Ocean Univ, Inst Marine Sci Technol & Environm, Dalian 116023, Peoples R China
2.Flinders Univ S Australia, Sch Med, Flinders Ctr Marine Bioprod Dev, Adelaide, SA 5042, Australia
3.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Adelaide, SA 5042, Australia
4.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
5.Key Lab Nearshore Marine Environm Sci & Technol L, Dalian 116023, Peoples R China
6.Dalian Ocean Univ, Grad Sch, Dalian 116023, Peoples R China
7.Dalian Ocean Univ, Vocat & Technol Coll, Dalian 116000, Peoples R China
8.Dalian Environm Monitoring Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Fu WanTao,Zhang JuLin,Zheng ChangBo,et al. Molecular cloning of partial 14-3-3 genes in the marine sponge Hymeniacidon perleve and its role in differentiating infectious and non-infectious bacteria[J]. CHINESE SCIENCE BULLETIN,2013,58(7):766-776.
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