中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Preparation and application of hollow molecularly imprinted polymers with a super-high selectivity to the template protein
作者: Chen, Yang1;  He, Xi-Wen1;  Mao, Jie1;  Li, Wen-You1;  Zhang, Yu-Kui1, 2, 3
关键词: Etching ;  Hollow core ;  Lysozyme ;  Molecularly imprinted polymers ;  Protein separation
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2013-10-01
DOI: 10.1002/jssc.201300709
卷: 36, 期:20, 页:3449-3456
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Protein-imprinted polymers with hollow cores that have a super-high imprinting factor were prepared by etching the core of the surface-imprinted polymers that used silica particles as the support. Lysozyme as template was modified onto the surface of silica particles by a covalent method, and after polymerization and the removal of template molecules, channels through the polymer layer were formed, which allowed a single-protein molecule to come into the hollow core and attach to the binding sites inside the polymer layer. The adsorption experiments demonstrated that the hollow imprinted polymers had an extremely high binding capacity and selectivity, and thus a super-high imprinting factor was obtained. The as-prepared imprinted polymers were used to separate the template lysozyme from egg white successfully, indicating its high selectivity and potential application in the field of separation of protein from real samples.
关键词[WOS]: SILICA NANOPARTICLES ;  SURFACE ;  RECOGNITION ;  ANTIBODIES ;  DOXORUBICIN ;  RECEPTORS ;  MEMBRANE ;  STRATEGY ;  CAPTURE
语种: 英语
WOS记录号: WOS:000325482500020
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137593
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Nankai Univ, Dept Chem, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Synerget Innovat Ctr Chem Sci & Engn Tianjin, Tianjin, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian, Peoples R China

Recommended Citation:
Chen, Yang,He, Xi-Wen,Mao, Jie,et al. Preparation and application of hollow molecularly imprinted polymers with a super-high selectivity to the template protein[J]. JOURNAL OF SEPARATION SCIENCE,2013,36(20):3449-3456.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Chen, Yang]'s Articles
 [He, Xi-Wen]'s Articles
 [Mao, Jie]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Chen, Yang]‘s Articles
 [He, Xi-Wen]‘s Articles
 [Mao, Jie]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace