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题名: A pH and temperature dual-responsive macroporous molecularly imprinted cryogel for enhanced recognition capability towards ovalbumin
作者: Gao, Feng-Xian1;  Zhao, Xiao-Li1;  He, Xi-Wen1;  Li, Wen-You1;  Zhang, Yu-Kui1, 2
刊名: ANALYTICAL METHODS
发表日期: 2013
DOI: 10.1039/c3ay41069b
卷: 5, 期:23, 页:6700-6708
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Chemistry, Analytical ;  Food Science & Technology ;  Spectroscopy
研究领域[WOS]: Chemistry ;  Food Science & Technology ;  Spectroscopy
英文摘要: A pH and temperature dual-responsive macroporous protein imprinted cryogel was synthesized by a facile "one-pot" method using N-isopropylacrylamide (NIPAAm) and 4-vinylphenylboronic acid (p-VPBA) as the main functional monomers. The smart molecularly imprinted polymers (MIPs) could recognize the target glycoprotein (ovalbumin (OB) as a template) dynamically and reversibly. The morphologies and features of the cryogels were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and differential scanning calorimetry (DSC). The rebinding and swelling experiments showed that the MIP cryogels could respond quickly to temperature and pH. Various parameters such as the pH value of the protein solution, adsorption temperature, and the rebinding time were optimized. Under the optimal conditions, a higher adsorption capacity and imprinting factor of the MIP cryogel were achieved, and were found to be 21 mg g(-1) and 3.4, respectively. Moreover, the MIP cryogel exhibited good selectivity and acceptable regeneration capacity. A real sample analysis further demonstrated its feasibility for the target protein separation. These excellent performances suggested that the obtained imprinted cryogel should be a promising candidate for recognition and separation of OB.
关键词[WOS]: BOVINE SERUM-ALBUMIN ;  SELECTIVE SEPARATION ;  PROTEIN ;  CHROMATOGRAPHY ;  GLYCOPEPTIDES ;  GLYCOPROTEINS ;  HYDROGEL ;  POLYMERS ;  GLYCOSYLATION ;  ANTIBODIES
语种: 英语
WOS记录号: WOS:000326946700024
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137646
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Gao, Feng-Xian,Zhao, Xiao-Li,He, Xi-Wen,et al. A pH and temperature dual-responsive macroporous molecularly imprinted cryogel for enhanced recognition capability towards ovalbumin[J]. ANALYTICAL METHODS,2013,5(23):6700-6708.
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