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A pH and temperature dual-responsive macroporous molecularly imprinted cryogel for enhanced recognition capability towards ovalbumin
Gao, Feng-Xian1; Zhao, Xiao-Li1; He, Xi-Wen1; Li, Wen-You1; Zhang, Yu-Kui1,2
Source PublicationANALYTICAL METHODS
2013
DOI10.1039/c3ay41069b
Volume5Issue:23Pages:6700-6708
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Life Sciences & Biomedicine ; Technology
WOS SubjectChemistry, Analytical ; Food Science & Technology ; Spectroscopy
WOS Research AreaChemistry ; Food Science & Technology ; Spectroscopy
WOS KeywordBOVINE SERUM-ALBUMIN ; SELECTIVE SEPARATION ; PROTEIN ; CHROMATOGRAPHY ; GLYCOPEPTIDES ; GLYCOPROTEINS ; HYDROGEL ; POLYMERS ; GLYCOSYLATION ; ANTIBODIES
AbstractA pH and temperature dual-responsive macroporous protein imprinted cryogel was synthesized by a facile "one-pot" method using N-isopropylacrylamide (NIPAAm) and 4-vinylphenylboronic acid (p-VPBA) as the main functional monomers. The smart molecularly imprinted polymers (MIPs) could recognize the target glycoprotein (ovalbumin (OB) as a template) dynamically and reversibly. The morphologies and features of the cryogels were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and differential scanning calorimetry (DSC). The rebinding and swelling experiments showed that the MIP cryogels could respond quickly to temperature and pH. Various parameters such as the pH value of the protein solution, adsorption temperature, and the rebinding time were optimized. Under the optimal conditions, a higher adsorption capacity and imprinting factor of the MIP cryogel were achieved, and were found to be 21 mg g(-1) and 3.4, respectively. Moreover, the MIP cryogel exhibited good selectivity and acceptable regeneration capacity. A real sample analysis further demonstrated its feasibility for the target protein separation. These excellent performances suggested that the obtained imprinted cryogel should be a promising candidate for recognition and separation of OB.
Language英语
WOS IDWOS:000326946700024
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/137646
Collection中国科学院大连化学物理研究所
Affiliation1.Nankai Univ, Dept Chem, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Gao, Feng-Xian,Zhao, Xiao-Li,He, Xi-Wen,et al. A pH and temperature dual-responsive macroporous molecularly imprinted cryogel for enhanced recognition capability towards ovalbumin[J]. ANALYTICAL METHODS,2013,5(23):6700-6708.
APA Gao, Feng-Xian,Zhao, Xiao-Li,He, Xi-Wen,Li, Wen-You,&Zhang, Yu-Kui.(2013).A pH and temperature dual-responsive macroporous molecularly imprinted cryogel for enhanced recognition capability towards ovalbumin.ANALYTICAL METHODS,5(23),6700-6708.
MLA Gao, Feng-Xian,et al."A pH and temperature dual-responsive macroporous molecularly imprinted cryogel for enhanced recognition capability towards ovalbumin".ANALYTICAL METHODS 5.23(2013):6700-6708.
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