中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Determination of Nucleosides in Escherichia coli by Rapid Resolution Liquid Chromatography-Tandem Quadrupole Mass Spectrometry
作者: Xie Yu-Ping1, 2;  Tian Jing1;  Gao Peng2;  Xu Guo-Wang2;  Fei Xu1;  Wang Yi1
关键词: Rapid resolution liquid chromatography-tandem mass spectrometry ;  Nucleoside ;  Escherichia coli
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2013
DOI: 10.3724/SP.J.1096.2013.20290
卷: 41, 期:1, 页:36-41
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A sensitive and selective method was developed for quantitation of nucleosides. The method included a UPLC HSS T3 column separation operated with a mobile phase of 10 mmol/L ammonium acetate and acetonitrile at a flow-rate of 0.2 mL/min coupled to a mass spectrometry scanned in multiple reaction monitoring (MRM) mode. In the result, the limits of quantification and detection of 19 nucleosides were in the range of 0.05-20 mu g/L and 0.02-10 mu g/L, respectively. The linearity of the detected nucleosides was excellent with R-2 > 0.99 and the limits of detection were all satisfied. The recoveries of standard additions for the compounds were between 79.01% and 119.76%, and the relative standard deviation was below 14%. The method was capable of quantitation for 7 nucleosides in Escherichia coli, of which guanosine was found to decrease along with culture phases.
关键词[WOS]: NUCLEOTIDE POOLS ;  GENOME
语种: 英语
WOS记录号: WOS:000315021700007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137649
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Dalian Polytech Univ, Sch Bioengn, Dalian 116034, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Xie Yu-Ping,Tian Jing,Gao Peng,et al. Determination of Nucleosides in Escherichia coli by Rapid Resolution Liquid Chromatography-Tandem Quadrupole Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2013,41(1):36-41.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Xie Yu-Ping]'s Articles
 [Tian Jing]'s Articles
 [Gao Peng]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Xie Yu-Ping]‘s Articles
 [Tian Jing]‘s Articles
 [Gao Peng]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace