DICP OpenIR
Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of MCAT from Synechocystis sp PCC 6803
Liu, Yinghui1; Zhang, Yanming2; Cao, Xupeng1; Xue, Song1
刊名ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
2013-11-01
DOI10.1107/S1744309113026274
69页:1256-1259
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics ; Crystallography
关键词[WOS]ACYL CARRIER PROTEIN ; CRYSTAL-STRUCTURE ; MALONYL-COA ; MYCOBACTERIUM-TUBERCULOSIS ; TRANSACYLASE ; MODELS ; SITE
英文摘要Malonyl-coenzymeA:acyl-carrier protein transacylase (MCAT), which catalyzes the transfer of the malonyl group from malonyl-CoA to acyl-carrier protein (ACP), is an essential enzyme in type II fatty-acid synthesis. The enzyme MCAT from Synechocystis sp. PCC 6803 (spMCAT), the first MCAT counterpart from a cyanobacterium, was cloned, purified and crystallized in order to determine its three-dimensional crystal structure. A higher-quality crystal with better diffraction was obtained by crystallization optimization. The crystal diffracted to 1.8 angstrom resolution and belonged to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 43.22, b = 149.21, c = 40.59 angstrom. Matthews coefficient calculations indicated that the crystal contained one spMCAT molecule in the asymmetric unit with a Matthews coefficient of 2.18 angstrom(3) Da(-1) and a solvent content of 43.65%.
语种英语
WOS记录号WOS:000326569300017
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/137655
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Agape Struct Solut, Surrey, BC V3W 0E9, Canada
推荐引用方式
GB/T 7714
Liu, Yinghui,Zhang, Yanming,Cao, Xupeng,et al. Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of MCAT from Synechocystis sp PCC 6803[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2013,69:1256-1259.
APA Liu, Yinghui,Zhang, Yanming,Cao, Xupeng,&Xue, Song.(2013).Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of MCAT from Synechocystis sp PCC 6803.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,69,1256-1259.
MLA Liu, Yinghui,et al."Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of MCAT from Synechocystis sp PCC 6803".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 69(2013):1256-1259.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Yinghui]的文章
[Zhang, Yanming]的文章
[Cao, Xupeng]的文章
百度学术
百度学术中相似的文章
[Liu, Yinghui]的文章
[Zhang, Yanming]的文章
[Cao, Xupeng]的文章
必应学术
必应学术中相似的文章
[Liu, Yinghui]的文章
[Zhang, Yanming]的文章
[Cao, Xupeng]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。