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题名: Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of MCAT from Synechocystis sp PCC 6803
作者: Liu, Yinghui1;  Zhang, Yanming2;  Cao, Xupeng1;  Xue, Song1
刊名: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
发表日期: 2013-11-01
DOI: 10.1107/S1744309113026274
卷: 69, 页:1256-1259
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology ;  Biophysics ;  Crystallography
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biophysics ;  Crystallography
英文摘要: Malonyl-coenzymeA:acyl-carrier protein transacylase (MCAT), which catalyzes the transfer of the malonyl group from malonyl-CoA to acyl-carrier protein (ACP), is an essential enzyme in type II fatty-acid synthesis. The enzyme MCAT from Synechocystis sp. PCC 6803 (spMCAT), the first MCAT counterpart from a cyanobacterium, was cloned, purified and crystallized in order to determine its three-dimensional crystal structure. A higher-quality crystal with better diffraction was obtained by crystallization optimization. The crystal diffracted to 1.8 angstrom resolution and belonged to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 43.22, b = 149.21, c = 40.59 angstrom. Matthews coefficient calculations indicated that the crystal contained one spMCAT molecule in the asymmetric unit with a Matthews coefficient of 2.18 angstrom(3) Da(-1) and a solvent content of 43.65%.
关键词[WOS]: ACYL CARRIER PROTEIN ;  CRYSTAL-STRUCTURE ;  MALONYL-COA ;  MYCOBACTERIUM-TUBERCULOSIS ;  TRANSACYLASE ;  MODELS ;  SITE
语种: 英语
WOS记录号: WOS:000326569300017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137655
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Agape Struct Solut, Surrey, BC V3W 0E9, Canada

Recommended Citation:
Liu, Yinghui,Zhang, Yanming,Cao, Xupeng,et al. Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of MCAT from Synechocystis sp PCC 6803[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2013,69:1256-1259.
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