DICP OpenIR
Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of MCAT from Synechocystis sp PCC 6803
Liu, Yinghui1; Zhang, Yanming2; Cao, Xupeng1; Xue, Song1
Source PublicationACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
2013-11-01
DOI10.1107/S1744309113026274
Volume69Pages:1256-1259
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics ; Crystallography
WOS KeywordACYL CARRIER PROTEIN ; CRYSTAL-STRUCTURE ; MALONYL-COA ; MYCOBACTERIUM-TUBERCULOSIS ; TRANSACYLASE ; MODELS ; SITE
AbstractMalonyl-coenzymeA:acyl-carrier protein transacylase (MCAT), which catalyzes the transfer of the malonyl group from malonyl-CoA to acyl-carrier protein (ACP), is an essential enzyme in type II fatty-acid synthesis. The enzyme MCAT from Synechocystis sp. PCC 6803 (spMCAT), the first MCAT counterpart from a cyanobacterium, was cloned, purified and crystallized in order to determine its three-dimensional crystal structure. A higher-quality crystal with better diffraction was obtained by crystallization optimization. The crystal diffracted to 1.8 angstrom resolution and belonged to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 43.22, b = 149.21, c = 40.59 angstrom. Matthews coefficient calculations indicated that the crystal contained one spMCAT molecule in the asymmetric unit with a Matthews coefficient of 2.18 angstrom(3) Da(-1) and a solvent content of 43.65%.
Language英语
WOS IDWOS:000326569300017
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/137655
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Agape Struct Solut, Surrey, BC V3W 0E9, Canada
Recommended Citation
GB/T 7714
Liu, Yinghui,Zhang, Yanming,Cao, Xupeng,et al. Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of MCAT from Synechocystis sp PCC 6803[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2013,69:1256-1259.
APA Liu, Yinghui,Zhang, Yanming,Cao, Xupeng,&Xue, Song.(2013).Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of MCAT from Synechocystis sp PCC 6803.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,69,1256-1259.
MLA Liu, Yinghui,et al."Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of MCAT from Synechocystis sp PCC 6803".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 69(2013):1256-1259.
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