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Two-dimensional strong cation exchange/positively charged reversed-phase liquid chromatography for alkaloid analysis and purification
Long, Zhen; Guo, Zhimou; Xue, Xingya; Zhang, Xiuli; Liang, Xinmiao
关键词Alkaloids Orthogonality Strong Cation Exchange Symmetrical Peaks
刊名JOURNAL OF SEPARATION SCIENCE
2013-12-01
DOI10.1002/jssc.201300863
36期:24页:3845-3852
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]W. T. WANG ; MASS-SPECTROMETRIC DETECTION ; BASIC COMPOUNDS ; STATIONARY-PHASE ; CAPILLARY-ELECTROPHORESIS ; SAMPLE PREPARATION ; SEPARATION ; PERFORMANCE ; HPLC ; EXTRACTION
英文摘要Peak tailing and nonalkaloid coelution usually hinder alkaloid purification. In this study, a 2DLC, strong cation exchange (SCX) coupled with positively charged RP (PGRP) LC, was developed to overcome these problems. Ten compounds including basic and nonbasic compounds were analyzed. Nonbasic compounds, which are coeluted with basic compounds on RP or PGRP columns, were weakly retained on the SCX column. In addition, a symmetrical peak shape (tailing factors <1.2) of basic compounds can be obtained in the current system. Compared to two other 2D systems, the current system provided the highest orthogonality (R-2 = 0.045). Furthermore, the SCX coupled with PGRP system was applied for alkaloid purification from a traditional Chinese medicine. Nineteen alkaloids were obtained and one of them was identified as a novel compound. The overall results demonstrate that the proposed system is a powerful tool for alkaloid purification.
语种英语
WOS记录号WOS:000328138200005
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被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/137665
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
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GB/T 7714
Long, Zhen,Guo, Zhimou,Xue, Xingya,et al. Two-dimensional strong cation exchange/positively charged reversed-phase liquid chromatography for alkaloid analysis and purification[J]. JOURNAL OF SEPARATION SCIENCE,2013,36(24):3845-3852.
APA Long, Zhen,Guo, Zhimou,Xue, Xingya,Zhang, Xiuli,&Liang, Xinmiao.(2013).Two-dimensional strong cation exchange/positively charged reversed-phase liquid chromatography for alkaloid analysis and purification.JOURNAL OF SEPARATION SCIENCE,36(24),3845-3852.
MLA Long, Zhen,et al."Two-dimensional strong cation exchange/positively charged reversed-phase liquid chromatography for alkaloid analysis and purification".JOURNAL OF SEPARATION SCIENCE 36.24(2013):3845-3852.
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