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题名: Two-dimensional strong cation exchange/positively charged reversed-phase liquid chromatography for alkaloid analysis and purification
作者: Long, Zhen1;  Guo, Zhimou1;  Xue, Xingya1;  Zhang, Xiuli1;  Liang, Xinmiao1
关键词: Alkaloids ;  Orthogonality ;  Strong cation exchange ;  Symmetrical peaks
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2013-12-01
DOI: 10.1002/jssc.201300863
卷: 36, 期:24, 页:3845-3852
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Peak tailing and nonalkaloid coelution usually hinder alkaloid purification. In this study, a 2DLC, strong cation exchange (SCX) coupled with positively charged RP (PGRP) LC, was developed to overcome these problems. Ten compounds including basic and nonbasic compounds were analyzed. Nonbasic compounds, which are coeluted with basic compounds on RP or PGRP columns, were weakly retained on the SCX column. In addition, a symmetrical peak shape (tailing factors <1.2) of basic compounds can be obtained in the current system. Compared to two other 2D systems, the current system provided the highest orthogonality (R-2 = 0.045). Furthermore, the SCX coupled with PGRP system was applied for alkaloid purification from a traditional Chinese medicine. Nineteen alkaloids were obtained and one of them was identified as a novel compound. The overall results demonstrate that the proposed system is a powerful tool for alkaloid purification.
关键词[WOS]: W. T. WANG ;  MASS-SPECTROMETRIC DETECTION ;  BASIC COMPOUNDS ;  STATIONARY-PHASE ;  CAPILLARY-ELECTROPHORESIS ;  SAMPLE PREPARATION ;  SEPARATION ;  PERFORMANCE ;  HPLC ;  EXTRACTION
语种: 英语
WOS记录号: WOS:000328138200005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137665
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China

Recommended Citation:
Long, Zhen,Guo, Zhimou,Xue, Xingya,et al. Two-dimensional strong cation exchange/positively charged reversed-phase liquid chromatography for alkaloid analysis and purification[J]. JOURNAL OF SEPARATION SCIENCE,2013,36(24):3845-3852.
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